Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China
Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China
复制标题
DOI：
10.1007/s00248-013-0272-6
复制
发表时间：
2013-08
影响因子：
3.6
通讯作者：
Jialin Li;Fuchao C. Li;Shu-xian Yu;S. Qin;Guangyi Wang
中科院分区：
文献类型：
--
作者：
Jialin Li;Fuchao C. Li;Shu-xian Yu;S. Qin;Guangyi Wang
海水养殖是全球主要的海产品供应之一，在沿海地区造成了严重的环境问题。它的快速发展扰乱了沉积物生态系统，从而影响了底栖细菌群落。采用基于16SRRNA基因的全长T-RFLP分析和克隆文库构建方法，对秦皇岛和大连沿海的邻近养殖场和非养殖区潮间带沉积物中的细菌多样性和群落结构进行了研究。丰富度和Shannon-Wiener指数在水产养殖场附近显著增加，从T-RFLP结果的峰值剖面来看，平均值分别为29和2.97。聚类分析表明，海水养殖对底泥细菌多样性的影响显著大于时间和空间尺度的影响。通过比较从所选择的5个样品的文库中获得的602个克隆的RFLP图谱，检索到137个OTU。所有文库都记录了γ-和δ-变形杆菌、芽孢杆菌、黄杆菌和放线杆菌的成员。此外，所有样品中以γ-变形杆菌为主(21.7%~45.0%)。冗余度分析表明，盐度、PO43−-P、NH4+-N和叶绿素含量决定了细菌组成的分布。γ-变形杆菌门、梭状芽孢杆菌门、黄杆菌门、芽孢杆菌门和植物霉菌门是造成克隆文库细菌差异的主要成分。我们的发现表明，这些门可以表现出与海水养殖造成的环境干扰有关的细菌组成的变化。
Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon–Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of γ- and δ-Proteobacteria,Bacilli,Flavobacteria, andActinobacteriawere recorded in all libraries. In addition,γ-Proteobacteriawere dominant in all samples (21.7~45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO43−-P, NH4+-N, and Chlorophyllacontent. The phyla of γ-Proteobacteria,Clostridia,Flavobacteria,Bacilli, andPlanctomyceteswere principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.