Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins
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DOI：
10.1016/s0014-5793(98)00440-2
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发表时间：
1998-05-08
期刊：
影响因子：
3.5
通讯作者：
Nagano, T
中科院分区：
文献类型：
--
作者：
Nakatsubo, N;Kojima, H;Nagano, T
一氧化氮（NO）的测定对于直接研究NO在各种生物系统中的调节作用具有重要意义。利用新型NO荧光指示剂二氨基荧光素（DAF）检测牛主动脉内皮细胞（EC）释放NO，DAF与NO反应生成相应的绿色荧光三唑荧光素，灵敏度和一个简单的协议直接检测NO。使用这些DAFs，我们可以检测NO的产生不仅从诱导型NO合酶表达的巨噬细胞，但也从组成型NO合酶表达的内皮细胞。(C)1998年欧洲生物化学学会联合会。
The measurement of nitric oxide (NO) is important for direct examination of the regulatory roles of NO in various biological systems, Diaminofluoresceins (DAFs), new fluorescence indicators for NO, were applied to detect the release of NO from bovine aortic endothelial cells (ECs), DAFs react with NO to yield the corresponding green-fluorescent triazolofluoresceins, which provide the advantages of specificity, sensitivity and a simple protocol for the direct detection of NO. Using these DAFs, we could detect the generation of NO not only from inducible NO synthase expressed in macrophages, but also from constitutive NO synthase expressed in ECs. (C) 1998 Federation of European Biochemical Societies.