Nucleotide sequence of Rhizobium meliloti 1021 nodulation genes: nodD is read divergently from nodABC.
Nucleotide sequence of Rhizobium meliloti 1021 nodulation genes: nodD is read divergently from nodABC.
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苜蓿根瘤菌1021结瘤基因的核苷酸序列：nodD与nodABC的读法不同。
DOI：
10.1089/dna.1985.4.241
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Long,SR
中科院分区：
文献类型：
--
作者：
Egelhoff,TT;Fisher,RF;Jacobs,TW;Mulligan,JT;Long,SR
结瘤(NOD)基因是根瘤菌入侵和刺激其寄主紫花苜蓿结瘤所必需的。我们已经建立了nodeD、noda和nodeB的DNA序列，它们是位于nifHDK下游20kb的基因簇的一部分。MelilotpSym巨质粒。308个氨基酸的开放阅读框(308个氨基酸)从HDK近端向HDK远端，从结点A(196个氨基酸)和结点B(217个氨基酸)偏离。这两个基因从HDK的远端向近端阅读，恰好位于先前定义的Node C开放阅读框架的上游。已对14个Tn5插入位点进行了测序，没有发现主要的插入热点，但总体上倾向于9-bp重复序列的第1和第9位的G/C碱基。
Nodulation (nod) genes are required forRhizobium melilotito invade and stimulate nodule formation in its host, alfalfa. We have established the DNA sequence ofnodD, nodA, andnodB, which are part of a gene cluster located 20 kb downstream ofnifHDKon theR. melilotipSym megaplasmid. ThenodDopen reading frame (308 amino acids) reads from proximal tonifHDKtoward distal tonifHDK, divergently fromnodA(196 aa) andnodB(217 aa). These two genes read from distal tonifHDKtoward proximal, and are just upstream from the previously defined open reading frame fornodC. Fourteen Tn5insertion sites have been sequenced innodD, nodA, andnodB, revealing no major hotspots for insertion, but an overall preference for G/C bases at positions 1 and 9 of the 9-bp repeat.