Role of pulse shape in cell membrane electropermeabilization
Role of pulse shape in cell membrane electropermeabilization
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DOI：
10.1016/s0005-2736(03)00173-1
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发表时间：
2003-08-07
影响因子：
3.4
通讯作者：
Mir, LM
中科院分区：
文献类型：
--
作者：
Kotnik, T;Pucihar, G;Mir, LM
单极矩形电脉冲的振幅、次数和持续时间在体外细胞膜电渗透中的作用已经成为几项研究的主题。对于单极矩形脉冲，已经报道了几种脉冲形状修改的效率提高：分离双极脉冲，连续双极波形和正弦调制脉冲。在本文中，我们提出了脉冲形状在渗透、细胞死亡和分子摄取中的作用的系统研究结果。我们首先比较了1毫秒单极脉冲与上升和下降时间范围从2到100 μ m的效率，观察到没有统计学上的显著差异。然后，我们比较了三角形、正弦和矩形双极脉冲的效率，最后比较了正弦调制单极脉冲在不同调制百分比下的效率。我们表明，这些实验的结果可以根据脉冲幅度超过某一临界值的时间来解释。(C) 2003 Elsevier B.V.版权所有
The role of the amplitude, number, and duration of unipolar rectangular electric pulses in cell membrane electropermeabilization in vitro has been the subject of several studies. With respect to unipolar rectangular pulses, an improved efficiency has been reported for several modifications of the pulse shape: separate bipolar pulses, continuous bipolar waveforms, and sine-modulated pulses. In this paper, we present the results of a systematic study of the role of pulse shape in permeabilization, cell death, and molecular uptake. We have first compared the efficiency of 1-ms unipolar pulses with rise- and falltimes ranging from 2 to 100 mus, observing no statistically significant difference. We then compared the efficiency of triangular, sine, and rectangular bipolar pulses, and finally the efficiency of sine-modulated unipolar pulses with different percentages of modulation. We show that the results of these experiments can be explained on the basis of the time during which the pulse amplitude exceeds a certain critical value. (C) 2003 Elsevier B.V. All rights reserved.