A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.
A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.
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DOI：
10.1105/tpc.9.6.895
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发表时间：
1997-06
期刊：
影响因子：
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通讯作者：
H. Weber;Ljudmilla Borisjuk;U. Heim;Norbert Sauer;U. Wobus
中科院分区：
文献类型：
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作者：
H. Weber;Ljudmilla Borisjuk;U. Heim;Norbert Sauer;U. Wobus
为了分析蚕豆种子发育过程中的糖转运过程，我们克隆了编码蔗糖和己糖转运蛋白的cDNA，分别命名为VfSUT1和VfSTP 1。在酵母中异源表达后证实了糖摄取活性。研究了基因表达与种子发育的关系。在营养和种子组织中均检测到转录本。在胚胎中，VfSUT1和VfSTP1 mRNA仅在表皮细胞中检测到，但在不同的时间和空间模式。VfSTP1 mRNA积累在子叶中期的表皮细胞覆盖的有丝分裂活跃的薄壁组织，而VfSUT1转录是特定的外表皮细胞显示转移细胞形态和覆盖的存储薄壁组织。传递细胞在子叶表皮和种皮的接触区域发育，首先在子叶早期开始，随后在子叶晚期扩展到远轴区域。喂食高浓度的糖抑制VfSUT1的表达和体外转移细胞分化，表明碳水化合物的可用性控制。
To analyze sugar transport processes during seed development of fava bean, we cloned cDNAs encoding one sucrose and one hexose transporter, designated VfSUT1 and VfSTP1, respectively. sugar uptake activity was confirmed after heterologous expression in yeast. Gene expression was studied in relation to seed development. Transcripts were detected in both vegetative and seed tissues. In the embryo, VfSUT1 and VfSTP1 mRNAs were detected only in epidermal cells, but in a different temporal and spatial pattern. VfSTP1 mRNA accumulates during the midcotyledon stage in epidermal cells covering the mitotically active parenchyma, whereas the VfSUT1 transcript was specific to outer epidermal cells showing transfer cell morphology and covering the storage parenchyma. Transfer cells developed at the contact area of the cotyledonary epidermis and the seed coat, starting first at the early cotyledon stage and subsequently spreading to the abaxial region at the late cotyledon stage. Feeding high concentrations of sugars suppressed both VfSUT1 expression and transfer cell differentiation in vitro, suggesting a control by carbohydrate availability.