RGS8 protein is distributed in dendrites and cell body of cerebellar Purkinje cell
RGS8 protein is distributed in dendrites and cell body of cerebellar Purkinje cell
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DOI：
10.1006/bbrc.2001.5489
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发表时间：
2001-09-14
影响因子：
3.1
通讯作者：
Saitoh, O
中科院分区：
文献类型：
--
作者：
Itoh, M;Odagiri, M;Saitoh, O
RGS8最初被鉴定为在神经元分化的P19细胞中特异性表达的RGS蛋白。我们使用合成肽产生了特异性针对大鼠RGS8的多克隆抗体。当非神经细胞（DDT1MF2，CHO，和NIH3T3）转染大鼠RGS8 cDNA免疫染色与此抗体，RGS8蛋白主要在细胞核中检测到。由于RGS8 mRNA仅在大鼠脑小脑的浦肯野细胞中表达，因此我们使用培养的小脑细胞和小脑组织切片进一步检查了RGS8蛋白在浦肯野细胞中的细胞分布。RGS8蛋白被排除在细胞核之外，分布在浦肯野细胞的胞体和树突中，但不在轴突中。这些结果表明存在一种控制RGS8蛋白在小脑浦肯野细胞中分布的机制。(C)北京：科学出版社.
RGS8 was originally identified as an RGS protein specifically expressed in neuronally differentiated P19 cells. We generated a polyclonal antibody specific to rat RGS8 using a synthetic peptide. When nonneural cells (DDT1MF2, CHO, and NIH3T3) transfected with rat RGS8 cDNA were immuno-stained with this antibody, the RGS8 protein was mainly detected in the nuclei. Since RGS8 mRNA was exclusively expressed in Purkinje cells of the cerebellum in the rat brain, we further examined the cellular distribution of the RGS8 protein in Purkinje cells using cultured cerebellar cells and tissue sections of the cerebellum. The RGS8 protein was excluded from the nuclei and distributed in the cell body and dendrites, but not in the axons of Purkinje cells. These results demonstrate the presence of a mechanism controlling the distribution of RGS8 protein in cerebellar Purkinje cells. (C) 2001 Academic Press.