Determining the Contents of Rupestonic Acid, Vitexicarpin, Apigenin, and Luteolin in Artemisia rupestris L. in Different Growth Stages by Thin-Layer Chromatographic Scanning
Determining the Contents of Rupestonic Acid, Vitexicarpin, Apigenin, and Luteolin in Artemisia rupestris L. in Different Growth Stages by Thin-Layer Chromatographic Scanning
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DOI：
10.1556/1006.2018.31.3.2
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发表时间：
2018-05
期刊：
影响因子：
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通讯作者：
Dongdong Lan;Jiajia Liu;Xiongfei Guo;Guoqiang. Zhu;S. Tian
中科院分区：
文献类型：
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作者：
Dongdong Lan;Jiajia Liu;Xiongfei Guo;Guoqiang. Zhu;S. Tian
采用薄层扫描法测定了不同生长阶段黄花蒿中4种成分的含量。展开剂为氯仿：甲醇：甲酸：水：6.35：0.63：0.17：0.07。温度为28-32℃，湿度小于30%。扫描波长分别为250 nm和352 nm。芦荟酸、牡荆素、芹菜素、木犀草素的线性范围分别为0.128~0.725、0.0706~0.400、0.0255~0.145、0.0172~0.0976μg。各组分的相关系数分别为0.9909、0.9908、0.9971、0.9934，呈良好的线性关系。精密度分析表明，相对标准偏差为5.0%。稳定性研究表明，芦荟酸、牡荆素、芹菜素和木犀草素在室温下至少可以稳定30分钟。平均回收率分别为99.9%、103.6%、98.07%和99.9%。对黄连不同生长阶段4种成分的含量进行了研究。这四种成分的明显变化可作为黄连药材质量控制和采收期确定的依据。
The contents of the four components in different growth stages of Artemisia rupestris L. was determined by thin-layer chromatographic scanning. Chloroform, methanol, formic acid, and water with the proportion of 6.35:0.63:0.17:0.07 were used as the developing solvent. The temperature was 28–32°C. The humidity was less than 30%. The scanning wavelengths were 250 nm and 352 nm. The linearity ranges of rupestonic acid, vitexicarpin, apigenin, and luteolin were 0.128–0.725, 0.0706–0.400, 0.0255–0.145, and 0.0172–0.0976 μg, respectively. The components had the corresponding correlation coefficients of 0.9909, 0.9908, 0.9971, and 0.9934, which showed a good linear relationship. Precision analyses showed a relative standard deviation of <5.0%. Stability studies showed that rupestonic acid, vitexicarpin, apigenin, and luteolin can be stable for at least 30 min at room temperature. Their average recovery percentages were 99.9%, 103.6%, 98.07%, and 99.9%, respectively. The contents of the four components in the different growth stages of A. rupestris L. were studied. The obvious changes of the four components could be used as the control for the quality of A. rupestris L. and the determination of the time of harvest.