DOES TRANEXAMIC ACID STABILISED FIBRIN SUPPORT THE OSTEOGENIC DIFFERENTIATION OF HUMAN PERIOSTEUM DERIVED CELLS?
DOES TRANEXAMIC ACID STABILISED FIBRIN SUPPORT THE OSTEOGENIC DIFFERENTIATION OF HUMAN PERIOSTEUM DERIVED CELLS?
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DOI：
10.22203/ecm.v021a21
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Van Oosterwyck, H.
中科院分区：
文献类型：
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作者：
Demol, J.;Eyckmans, J.;Van Oosterwyck, H.
长期以来，纤维蛋白封闭剂一直被用作成骨细胞在骨再生中的载体。然而，目前还没有证明纤维蛋白的作用是否仅限于将细胞输送到骨缺损处，或者纤维蛋白是否促进了成骨。本研究研究了纤维蛋白对体外培养的人骨膜衍生细胞(HPDCs)在二维(纤维蛋白基质)和三维(纤维蛋白载体)环境中成骨条件下行为的影响。在研究氨甲环酸(TEA)对塑料单层培养人脐静脉内皮细胞(HPDCs)的影响后，研究了其对纤维蛋白降解的抑制作用。在此条件下，TEA不影响hPDCs的增殖和钙沉积。除了碱性磷酸酶(ALP)表达降低(第14天)外，在TEA存在的情况下，特定成骨标记物的表达谱也保持不变。与塑料相比，到第21天，2D纤维蛋白底物的DNA含量高出220%，促进了细胞的增殖。基因表达也发生了显著变化(p<0.05)。
Fibrin sealants have long been used as carrier for osteogenic cells in bone regeneration. However, it has not been demonstrated whether fibrin's role is limited to delivering cells to the bone defect or whether fibrin enhances osteogenesis. This study investigated fibrin's influence on the behaviour of human periosteum-derived cells (hPDCs) when cultured in vitro under osteogenic conditions in two-dimensional (fibrin substrate) and three-dimensional (fibrin carrier) environments. Tranexamic acid (TEA) was used to reduce fibrin degradation after investigating its effect on hPDCs in monolayer culture on plastic. TEA did not affect proliferation nor calcium deposition of hPDCs under these conditions. Expression profiles of specific osteogenic markers were also maintained within the presence of TEA, apart from reduced alkaline phosphatase (ALP) expression (day 14). Compared to plastic, proliferation was upregulated on 2D fibrin substrates with a 220% higher DNA content by day 21. Gene expression was also altered, with significantly (p