Optical-fiber-microsphere for remote fluorescence correlation spectroscopy
Optical-fiber-microsphere for remote fluorescence correlation spectroscopy
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DOI：
10.1364/oe.17.019085
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发表时间：
2009-10-21
期刊：
影响因子：
3.8
通讯作者：
Rigneault, Herve
中科院分区：
文献类型：
--
作者：
Aouani, Heykel;Deiss, Frederique;Rigneault, Herve
荧光相关光谱（FCS）是一种通用的方法，将大大有利于远程光纤荧光传感器。然而，目前的最先进的斗争与高背景和低检测灵敏度，防止基于光纤的FCS扩展到单分子水平。在这里，我们报告使用的光纤结合乳胶微球进行FCS分析。由于光子纳米喷射效应，该技术的灵敏度在单分子水平上得到了证明。这为减少笨重的显微镜设置和将FCS扩展到远程或体内应用提供了新的机会。(C)2009年美国光学学会
Fluorescence correlation spectroscopy (FCS) is a versatile method that would greatly benefit to remote optical-fiber fluorescence sensors. However, the current state-of-the-art struggles with high background and low detection sensitivities that prevent the extension of fiber-based FCS down to the single-molecule level. Here we report the use of an optical fiber combined with a latex microsphere to perform FCS analysis. The sensitivity of the technique is demonstrated at the single molecule level thanks to a photonic nanojet effect. This offers new opportunities for reducing the bulky microscope setup and extending FCS to remote or in vivo applications. (C) 2009 Optical Society of America