Functional significance of myosin transitions in single fibers of developing soleus muscle.
Functional significance of myosin transitions in single fibers of developing soleus muscle.
复制标题
发育中比目鱼肌单纤维中肌球蛋白转变的功能意义。
DOI：
10.1152/ajpcell.1988.254.5.c605
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Moss,RL
中科院分区：
文献类型：
--
作者：
Reiser,PJ;Kasper,CE;Greaser,ML;Moss,RL
对来自新生和成年大鼠比目鱼肌的单个化学剥皮纤维的最大缩短速度和肌球蛋白重链（MHC）组成进行了检查，以确定大鼠慢速肌肉发育过程中这些参数之间的关系。此外，还研究了同龄比目鱼肌纤维束的 MHC 组成。使用十二烷基硫酸钠(SDS)-聚丙烯酰胺凝胶电泳检查MHC组合物。纤维束的结果表明，从出生后 3 天到 5 个月，大鼠比目鱼肌主要含有从成年慢肌迁移到 MHC 附近的 MHC。从出生后 14 天到 2 个月，还有大量额外的 MHC 在 SDS 凝胶上与成年大鼠快肌的特征共迁移。所有在产后 3 天和 7 天以及 5 个月研究的纤维以及产后 14 天至 2 个月的大多数纤维具有相对较低的缩短速度。后一组的一些纤维具有明显更高的速度。每个年龄的快速纤维具有更多的 MHC，这些 MHC 与 SDS 凝胶上的成人快速型 MHC 共迁移。因此，大鼠比目鱼肌单纤维的缩短速度似乎与出生后发育过程中的 MHC 组成有关。
The maximal velocity of shortening and myosin heavy chain (MHC) composition of single, chemically skinned fibers from neonatal and adult rat soleus muscles were examined to determine the relationship between these parameters during slow muscle development in the rat. In addition, the MHC composition of bundles of fibers from soleus muscles at the same ages was studied. The MHC compositions were examined using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The results from the bundles of fibers indicate that from 3 days to 5 mo postnatal, the rat soleus contains predominantly MHCs that migrate in the vicinity of the MHC from adult slow muscle. From 14 days to 2 mo postnatal, there are also significant amounts of additional MHCs that comigrate on SDS gels with those characteristic of adult rat fast muscle. All the fibers studied at 3 and 7 days postnatal and at 5 mo and the majority of fibers from 14 days to 2 mo postnatal had relatively low shortening velocities. A few fibers from the latter group had significantly higher velocities. The faster fibers at each age had greater amounts of the MHCs that comigrate with the adult fast-type MHC on SDS gels. Thus the velocity of shortening of single fibers from the rat soleus muscle appears to be related to MHC composition during postnatal development.