Structural, Thermal Stability and In Vivo Bioactivity Properties of Nanobioglasses Containing ZnO
Structural, Thermal Stability and In Vivo Bioactivity Properties of Nanobioglasses Containing ZnO
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DOI：
10.1166/sam.2019.3554
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发表时间：
2019-07-01
影响因子：
0.9
通讯作者：
Yousef, El Sayed
中科院分区：
文献类型：
--
作者：
Algarni, H.;AlShahrani, Ibrahim;Yousef, El Sayed
本文采用淬火熔体法制备了40P(2)O(5)-20Na(2)O- 10ca (OH)(2)-20CaCl(2)-10ZnO（标记BGZn）的新型纳米生物玻璃。利用差热分析（DTA）、x射线衍射（XRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和拉曼光谱对制备的玻璃进行了热学和微观结构研究。我们测试了BGZn的抗菌活性、体外对正常和活化脾细胞的抗增殖/细胞毒性、体内免疫反应和超敏反应、体内和体外细胞毒性作用以及活化脾细胞对BGZn的体外作用。结果表明，BGZn（纳米颗粒粉末）对革兰氏阴性和阳性细菌以及真菌具有抗菌活性。纳米颗粒的抑菌活性强于粉末。BGZn粉在高浓度时对正常脾细胞具有细胞毒作用，在低稀释时具有刺激作用，而纳米颗粒则具有抑制/细胞毒作用。BGZn粉对活化的脾细胞无明显影响。体内试验表明，BGZn是一种惰性物质，不刺激免疫系统，不引起局部反应。活化的脾细胞产物未引起BGZn结构的改变。BGZn对红细胞没有任何急性细胞毒性或溶解作用。最后，BGZn是一种惰性物质，不引起免疫刺激或肝细胞毒性。BGZn不受免疫细胞裂解产物的影响。BGZn是一种安全、理想的生物体内植入材料。
In the present paper we prepared novel nanobioglasses with composition 40P(2)O(5)-20Na(2)O-10Ca(OH)(2)-20CaCl(2)-10ZnO in mol% (labeled BGZn) by using quenching melt fabrication. We investigate the thermal and microstructural of prepared glasses by using differential thermal analysis (DTA), X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and Raman spectra. We were tested for its antimicrobial activity, anti-proliferative/cytotoxicity against normal and activated splenic cells in vitro, in vivo immunologic and hypersensitivity responses, in vivo and in vitro cytotoxicity effects and in vitro effects of activated splenic cells on BGZn Results showed that BGZn (powder with nanoparticles) had antimicrobial activities against Gramnegative and positive bacteria as well as fungi. The antimicrobial activity of nanoparticles was stronger than the powder. BGZn powder demonstrated the cytotoxic effect on normal splenic cells at high concentrations and stimulatory effect at low dilution, while nanoparticles showed inhibitory/cytotoxic effects. BGZn powder showed no effects on activated splenic cells. In vivo test demonstrated that BGZn is an inert material which did not stimulate the immune system or caused local reactions. The products of activated splenic cells did not cause any changes in the structure of BGZn. BGZn did not cause any acute cytotoxicity or lysis to RBCs. Finally BGZn is an inert material which did not cause immune stimulation or hepatic cytotoxicity. BGZn was not affected by lytic products of immune cells. BGZn can be considered as a safe and ideal material for implantation inside the body of living organisms.