A simple, rapid, efficient method for the preparation of gamma 32P-labeled guanosine triphosphate (GTP) and adenosine triphosphate (ATP).
A simple, rapid, efficient method for the preparation of gamma 32P-labeled guanosine triphosphate (GTP) and adenosine triphosphate (ATP).
复制标题
一种简单、快速、高效的制备 γ32P 标记的三磷酸鸟苷 (GTP) 和三磷酸腺苷 (ATP) 的方法。
DOI：
10.1016/s0021-9258(19)70249-x
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
E. Guinto
中科院分区：
文献类型：
--
作者：
E. Beutler;E. Guinto
A simplified new method for synthesis fo gamma 32P GTP and gamma 32P ATP is described. Radiophosphorus is incorporated into GDP and ADP to form GTP and ATP, respectively, in the phosphoglycerate kinase reaction. The product is separated by ion exchange resin chromatography. The procedure is simple, gives a product a high purity, and the label is confined to the gamma position.
