Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization
Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization
复制标题
DOI：
10.1177/030098589803500212
复制
发表时间：
1998-03-01
影响因子：
2.4
通讯作者：
Jorsal, SE
中科院分区：
文献类型：
--
作者：
Boye, M;Jensen, TK;Jorsal, SE
靶向16S核糖体RNA的荧光原位杂交被用于特异性检测的专性细胞内细菌胞内劳森氏菌肠上皮细胞从猪受增殖性肠病。设计了一种特异性寡核苷酸探针，并与间接免疫荧光法进行了比较，确定了探针的特异性。在福尔马林固定的组织样品中，来自15只受猪增殖性肠病影响的猪的细胞内细胞。我们使用了10个来自没有增殖性粘膜变化的猪的组织样本作为阴性对照。结果表明，该寡核苷酸探针对L.以核糖体RNA为靶点荧光原位杂交技术是一种快速、特异检测和组织学识别L.福尔马林固定的组织中的细胞内。
Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.