The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.
The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.
复制标题
果蝇毛翼蛋白的抑制因子与吉普赛反转录转座子的特定序列结合。
DOI：
10.1101/gad.2.11.1414
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发表时间：
1988
影响因子：
10.5
通讯作者：
Corces,VG
中科院分区：
文献类型：
--
作者：
Spana,C;Harrison,DA;Corces,VG
黑腹果蝇Hairy-wing [su（Hw），3-54.8]基因座抑制子突变可逆转吉普赛转座因子诱导的第二位点突变的表型。该基因编码一种蛋白质，预测分子量为109，000，含有一个酸性结构域和12个拷贝的DNA结合“锌指”基序。su（Hw）蛋白在大肠杆菌和果蝇细胞中过表达，并得到部分纯化。它被证明在体外与一个367 bp的DNA片段特异性相互作用，该片段含有12个拷贝的PyPuTTGCATACCPy序列，位于吉普赛人的5 '-非翻译区，在5'长末端重复序列（LTR）和第一个ATG起始密码子之间。这个序列显示出惊人的同源性，一些哺乳动物的转录增强子元件，支持的su（Hw）蛋白在吉普赛转录控制的作用。此外，su（Hw）蛋白存在于果蝇多线染色体上约100-200个位点，表明它也与DNA在体内相互作用，并可能在功能上参与正常细胞基因的调节。
Mutations at the suppressor of Hairy-wing [su(Hw), 3-54.8] locus reverse the phenotype of second-site mutations induced by the gypsy transposable element in Drosophila melanogaster. This gene encodes a protein with a predicted molecular weight of 109,000 that contains an acidic domain and 12 copies of the DNA-binding 'Zn finger' motif. The su(Hw) protein was overexpressed in Escherichia coli and Drosophila cells, and partially purified. It was shown to interact specifically in vitro with a 367-bp DNA fragment that contains 12 copies of the sequence PyPuTTGCATACCPy located in the 5'-untranslated region of gypsy, between the 5' long terminal repeat (LTR) and the first ATG initiation codon. This sequence shows striking homology to some mammalian transcriptional enhancer elements, supporting a role for the su(Hw) protein in the control of gypsy transcription. In addition, the su(Hw) protein is present at approximately 100-200 sites on Drosophila polytene chromosomes, suggesting that it also interacts in vivo with DNA and might be involved functionally in the regulation of normal cellular genes.