Articular-cartilage matrix gamma-carboxyglutamic acid-containing protein. Characterization and immunolocalization.
Articular-cartilage matrix gamma-carboxyglutamic acid-containing protein. Characterization and immunolocalization.
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关节软骨基质含γ-羧基谷氨酸的蛋白质。
DOI：
10.1042/bj2820001
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Wallin,R
中科院分区：
文献类型：
--
作者：
Loeser,R;Carlson,CS;Tulli,H;Jerome,WG;Miller,L;Wallin,R
通过对人和牛软骨氯化胍提取物的Western-Blot分析，发现含有基质γ-羧谷氨酸(GLA)的蛋白(MGP)存在于关节软骨中，并通过对人和猴子标本的免疫组织化学研究进一步定位。在新生关节软骨中，MGP弥漫分布于整个基质中，而在生长板软骨中，MGP主要见于晚期肥大和钙化带软骨细胞。在成人关节软骨中，MGP主要存在于软骨细胞和细胞周围基质中。免疫电子显微镜研究显示，MGP与泡状结构之间存在关联，其外观与基质小泡一致。MGP可能是软骨钙化的重要调节因子，因为它在软骨中的定位以及已知的含有GLA的蛋白与钙和羟基磷灰石的亲和力。
Matrix gamma-carboxyglutamic acid (Gla)-containing protein (MGP) was found to be present in articular cartilage by Western-blot analysis of guanidinium chloride extracts of human and bovine cartilage and was further localized by immunohistochemical studies on human and monkey specimens. In newborn articular cartilage MGP was present diffusely throughout the matrix, whereas in growth-plate cartilage it was seen mainly in late hypertrophic and calcifying-zone chondrocytes. In adult articular cartilage MGP was present primarily in chondrocytes and the pericellular matrix. Immunoelectron microscopy studies revealed an association between MGP and vesicular structures with an appearance consistent with matrix vesicles. MGP may be an important regulator of cartilage calcification because of its localization in cartilage and the known affinity of Gla-containing proteins for Ca2+ and hydroxyapatite.