Friend murine leukemia virus-immortalized myeloid cells are converted into tumorigenic cell lines by Abelson leukemia virus.
Friend murine leukemia virus-immortalized myeloid cells are converted into tumorigenic cell lines by Abelson leukemia virus.
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Friend鼠白血病病毒永生化骨髓细胞被Abelson白血病病毒转化为致瘤细胞系。
DOI：
10.1073/pnas.82.10.3306
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发表时间：
1985
影响因子：
11.1
通讯作者：
Bauchwitz,R
中科院分区：
文献类型：
--
作者：
Oliff,A;Agranovsky,O;McKinney,MD;Murty,VV;Bauchwitz,R
Friend鼠白血病病毒（Fr-MuLV）是一种可复制的鼠逆转录病毒，可在NFS/n小鼠中诱导急性非淋巴细胞白血病。Fr-MuLV疾病根据白血病细胞在培养物中生长和移植到同基因小鼠中的能力分为两个阶段。从Fr-MuLV感染后的疾病早期取得的造血细胞在WEHI-3细胞条件培养基（CM）或白细胞介素3的存在下作为永生骨髓细胞系生长。这些生长因子依赖性细胞系在不存在CM的情况下在培养物中不生长，并且在同基因动物中不形成肿瘤。如果这些Fr-MuLV感染的细胞与Abelson鼠白血病病毒（Ab-MuLV）重叠感染，它们将失去对WEHI-3 CM的依赖性，并在缺乏外源性生长因子的情况下在培养物中增殖。伴随着培养物中生长因子依赖性的丧失，Ab-MuLV感染的细胞系在同系小鼠中成为致瘤性的。这种二级转化是Ab-MuLV特异性的。用哈维鼠肉瘤病毒（Ha-MuSV）或嗜酸性病毒超感染的Fr-MuLV-永生化骨髓细胞系在体外生长仍然依赖于WEHI-3 CM，并且在体内不具有致瘤性。无论是Ab-MuLV，也不是Ha-MuSV感染的正常小鼠骨髓细胞培养产生生长因子非依赖性或致瘤细胞系。我们的结论是，至少需要两个遗传事件转换成致瘤细胞系的鼠骨髓前体。第一个事件发生在Fr-MuLV感染的小鼠中，产生生长因子依赖性但在体外永生的细胞。第二个事件可以通过Ab-MuLV感染完成，将这些永生的骨髓前体转化为生长因子非依赖性和致瘤性细胞。
Friend murine leukemia virus (Fr-MuLV) is a replication-competent murine retrovirus that induces acute nonlymphocytic leukemias in NFS/n mice. Fr-MuLV disease is divided into two stages based on the ability of the leukemia cells to grow in culture and transplant into syngeneic mice. Hematopoietic cells taken from the early stage of disease after Fr-MuLV infection grow as immortal myeloid cell lines in the presence of WEHI-3 cell-conditioned medium (CM) or interleukin 3. These growth factor-dependent cell lines do not grow in culture in the absence of CM and do not form tumors in syngeneic animals. If these Fr-MuLV-infected cells are superinfected with Abelson murine leukemia virus (Ab-MuLV), they lose their dependence on WEHI-3 CM and proliferate in culture in the absence of exogenous growth factors. Concomitant with the loss of growth factor dependence in culture, the Ab-MuLV-infected cell lines become tumorigenic in syngeneic mice. This secondary level of transformation is Ab-MuLV specific. Fr-MuLV-immortalized myeloid cell lines superinfected with Harvey murine sarcoma virus (Ha-MuSV) or amphotropic virus remain dependent on WEHI-3 CM for growth in vitro and are not tumorigenic in vivo. Neither Ab-MuLV- nor Ha-MuSV-infected normal mouse myeloid cell cultures produce growth factor-independent or tumorigenic cell lines. We conclude that at least two genetic events are needed to convert a murine myeloid precursor into a tumorigenic cell line. The first event occurs in Fr-MuLV-infected mice, generating cells that are growth factor dependent but immortal in vitro. The second event, which can be accomplished by Ab-MuLV infection, converts these immortal myeloid precursors into growth factor-independent and tumorigenic cells.