Pressure and angiotensin II synergistically induce aortic fibronectin expression in organ culture model of rabbit aorta - Evidence for a pressure-induced tissue renin-angiotensin system
Pressure and angiotensin II synergistically induce aortic fibronectin expression in organ culture model of rabbit aorta - Evidence for a pressure-induced tissue renin-angiotensin system
复制标题
DOI：
10.1161/01.res.79.1.70
复制
发表时间：
1996-07-01
影响因子：
20.1
通讯作者：
Tedgui, A
中科院分区：
文献类型：
--
作者：
Bardy, N;Merval, R;Tedgui, A
主动脉纤维连接蛋白（FN）在高血压中表达增强。越来越多的证据表明，血管紧张素II （Ang II）和机械因素都可能诱导高血压患者的血管重构。我们之前的研究表明，在体外，跨壁压力的增加增强了兔主动脉介质中FN的表达。为了研究压力作用与Ang II之间的联系，并探索这种关系的机制，我们使用免疫标记分析量化了Ang II和Ang II抑制剂对兔主动脉3天器官培养模型中压力依赖性FN表达的影响，并通过原位杂交检测FN mrna。在80和150 mm Hg时，Ang II对FN表达有剂量依赖性影响，而在0 mm Hg（松弛血管）时则没有。1 μ mol/L Ang使培养基横截面表面积增加，在80 mm Hg下FN表达从7.9+/-0.7% （n=9）增加到18.9+/-1.1% （n=4）
Aortic fibronectin (FN) expression is augmented in hypertension. Increasing evidence suggests that both angiotensin II (Ang II and mechanical factors may induce vascular remodeling in response to hypertension. We have previously shown that, in vitro, increased transmural pressure enhances FN expression in rabbit aortic media. To investigate the existence of a link between the effects of pressure and Ang II and to explore the mechanisms underlying such a relationship, we quantified the effect of Ang II and Ang II inhibitors on the pressure-dependent FN expression in a 3-day organ culture model of rabbit aorta using immunolabeling analysis and detected FN mRNAs by in situ hybridization. A dose-dependent effect of Ang II on FN expression was observed at both 80 and 150 mm Hg but not at 0 mm Hg (relaxed vessels). One mu mol/L Ang II increased the media cross-sectional surface, showing FN expression from 7.9+/-0.7% (n=9) to 18.9+/-1.1% (n=4) at 80 mm Hg (P