Study on spoilage potential and its molecular basis of Shewanella putrefaciens in response to cold conditions by Label-free quantitative proteomic analysis
Study on spoilage potential and its molecular basis of Shewanella putrefaciens in response to cold conditions by Label-free quantitative proteomic analysis
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DOI：
10.1007/s11274-022-03479-y
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发表时间：
2022-12
影响因子：
4.1
通讯作者：
Y. Qian;Ying Cheng;Jing Xie;S. Yang
中科院分区：
文献类型：
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作者：
Y. Qian;Ying Cheng;Jing Xie;S. Yang
为了阐明希瓦氏菌如何在寒冷条件下存活并产生腐败产物，研究了希瓦氏菌的代谢和蛋白酶活性。采用Label-free定量蛋白质组学分析方法，对在30°C、10°C和4°C三种不同温度下培养的putrefaciensDSM6067进行细菌生长、总挥发性碱性氮（tbn - n）、生物胺、胞外蛋白酶活性以及差异表达蛋白的测定。菌株在10°C和4°C条件下的滞后期比在30°C条件下分别延长约20 h和120 h。在30℃条件下，TVB-N在28 h内增加到89.23 mg N/100 g，在10℃和4℃条件下，TVB-N分别至少需要72 h和224 h。低温（10°C和4°C）也抑制了滞后期产率因子和细胞外蛋白酶活性。然而，在10°C和4°C培养的样品的每细胞蛋白酶活性和产量因子都恢复得很好，特别是在log期的中后期。进一步的定量蛋白质组学分析显示了应对冷胁迫的复杂生物网络：冷胁迫反应、营养吸收和节能策略。观察到蛋白酶和肽酶的上调，从而影响了丝氨酸、精氨酸和天冬氨酸的降解途径，这可能导致腐败产物的积累。本研究突出了s。即使在低温下，也要注意腐臭。
To elucidate howShewanella putrefacienssurvives and produces spoilage products in response to cold conditions, the metabolic and protease activity ofS. putrefaciensDSM6067 cultured at three different temperatures (30 °C, 10 °C, and 4 °C) was studied by determining the bacterial growth, total volatile basic nitrogen (TVB-N), biogenic amines, extracellular protease activity, as well as the differential expressed proteins via Label-free quantitative proteomics analysis. The lag phase of the strain cultured at 10 °C and 4 °C was about 20 h and 120 h longer than at 30 °C, respectively. The TVB-N increased to 89.23 mg N/100 g within 28 h at 30 °C, and it needed at least 72 h and 224 h at 10 °C and 4 °C, respectively. Cold temperatures (10 °C and 4 °C) also inhibited the yield factors and the extracellular protease activity per cell at the lag phase. However, the protease activity per cell and the yield factors of the sample cultivated at 10 °C and 4 °C well recovered, especially at the mid and latter stages of the log phase. The further quantitative proteomic analysis displayed a complex biological network to tackle cold stress: cold stress responses, nutrient uptake, and energy conservation strategy. It was observed that the protease and peptidase were upregulated, so as to the degradation pathways of serine, arginine, and aspartate, which might lead to the accumulation of spoilage products. This study highlighted the spoilage potential ofS. putrefaciensstill should be concerned even at low temperatures.