PROGRAMMED CELL-DEATH AND EXPRESSION OF THE PROTOONCOGENE BCL-2 IN MYOCYTES DURING POSTNATAL MATURATION OF THE HEART
PROGRAMMED CELL-DEATH AND EXPRESSION OF THE PROTOONCOGENE BCL-2 IN MYOCYTES DURING POSTNATAL MATURATION OF THE HEART
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DOI：
10.1006/excr.1995.1211
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发表时间：
1995-07-01
影响因子：
3.7
通讯作者：
ANVERSA, P
中科院分区：
文献类型：
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作者：
KAJSTURA, J;MANSUKHANI, M;ANVERSA, P
为了确定程序性肌细胞死亡是否是发育过程中左右心室差异性生长适应的主要组成部分，在妊娠结束时和出生后1、5、11和21天的大鼠心脏中鉴定并定量了肌细胞核中DNA链断裂的形成。还评价了BrdU在左心室和右心室肌细胞中的掺入。同时检测心肌细胞bcl-2的表达。程序性心肌细胞死亡是缺席的胎儿心脏，但影响心肌出生后。这种现象在21天时不再可检测到。非肌细胞核中的DNA链断裂存在于所有时间间隔。在右心室、室间隔和左心室中，1天时分别有10.4、6.1和2.5个肌细胞核/10，000个核出现DNA降解。第5天的相应值分别为3.7、3.5和2.0个肌细胞核/10，000个核。在第11天，在心脏的这三个区域中，程序性细胞死亡涉及1.2、1.5和0.53个肌细胞核/10，000个核。在第1、5和11天，右心室中程序性细胞死亡的幅度分别增加了4.2倍、1.9倍和2.3倍，具有统计学显著性。与此相反，BrdU掺入在心肌细胞中以可比的方式在左心室和右心室成熟。重要的是，bcl-2 mRNA水平高，在胎儿心肌细胞，显着下降，在1和5天，并逐步增加，在II和21天。bcl-2在右心室肌细胞的表达低于左心室肌细胞。总之，程序性心肌细胞死亡与bcl-2表达呈负相关，并且在出生后发育过程中对右心室的影响大于左心室。这种现象可能是至关重要的调制过程中的心肌细胞的数量在两个心室从胎儿到成人的循环系统的过渡。(C)出版社：Academic Press
To determine whether programmed myocyte cell death is a major component of the differential growth adaptation of the right and left ventricle during development, the formation of DNA strand breaks in myocyte nuclei was identified and quantitated in hearts of rats at the end of gestation and at 1, 5, 11, and 21 days after birth. Incorporation of BrdU in left and right ventricular myocytes was also evaluated. Moreover, the expression of bcl-2 in myocytes was determined. Programmed myocyte cell death was absent in the fetal heart but affected the myocardium postnatally. This phenomenon was no longer detectable at 21 days. DNA strand breaks in nonmyocyte nuclei were present at all time intervals. Quantitatively, 10.4, 6.1, and 2.5 myocyte nuclei/10,000 nuclei exhibited DNA degradation at 1 day in the right ventricle, interventricular septum, and left ventricle, respectively. Corresponding values at 5 days were 3.7, 3.5, and 2.0 myocyte nuclei/10,000 nuclei. At 11 days, programmed cell death involved 1.2, 1.5, and 0.53 myocyte nuclei/10,000 nuclei in these three regions of the heart. The 4.2-fold, 1.9-fold, and 2.3-fold greater magnitude of programmed cell death in the right ventricle at 1, 5, and 11 days was statistically significant. In contrast, BrdU incorporation in myocytes decreased in a comparable manner in the left and right ventricles with maturation. Importantly, bcl-2 mRNA levels were high in fetal myocytes, decreased markedly at 1 and 5 days, and progressively increased at II and 21 days. The expression of bcl-2 was lower in right than in left ventricular myocytes. In conclusion, programmed myocyte cell death is inversely related to bcl-2 expression and affects the right ventricle more than the left ventricle during postnatal development. This phenomenon may be crucial in the modulation of the number of myocytes in the two ventricles during the transition from the fetal to the adult circulatory system. (C) 1995 Academic Press, Inc.