Tissue Distribution Comparison of Six Active Ingredients from an Eucommiae Cortex Extract between Normal and Spontaneously Hypertensive Rats
Tissue Distribution Comparison of Six Active Ingredients from an Eucommiae Cortex Extract between Normal and Spontaneously Hypertensive Rats
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杜仲提取物中的六种活性成分在正常和自发性高血压大鼠的组织分布比较
DOI：
10.1155/2020/2049059
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发表时间：
2020-06-08
影响因子：
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通讯作者：
Gong, ZiPeng
中科院分区：
文献类型：
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作者：
Hu, Hejia;Xiao, Hongqin;Gong, ZiPeng
杜仲是一种名贵滋补中药，对高血压有良好的治疗作用。本研究比较了EC提取物的6种代表性活性成分京尼平酸（GA）、原儿茶酸（PCA）、新绿原酸（NCA）、绿原酸（CA）、（+）-松醇二-O-β-D-吡喃葡萄糖苷（PDG）和（+）-松醇4 '-O-β-D-吡喃葡萄糖苷（PG）在正常大鼠和自发性高血压大鼠（SHR）的组织分布。每只大鼠灌胃给予EC提取物，剂量为5.4 g/kg。在给药后10 min、30 min、2 h和8 h处死大鼠;每个时间点处死6只大鼠。然后，我们很快就收获了他们的主要器官，包括心脏，肝脏，脾脏，肺，肾脏和大脑。采用超高效液相色谱-串联质谱法（UPLC-MS/MS）测定上述6种成分在两种大鼠脏器中的含量，并分析其组织分布的差异。结果表明，SHR与正常组在各时间点的各组分水平均存在差异。随着时间的推移，GA在SHR各组织中的分布与正常组不同的器官逐渐增多：SHR的GA水平低于正常大鼠。两组在10和30分钟时的PG和PDG水平相似。SHR组与正常组在10 min、30 min、2 h的NCA和CA也有一定的相似性。结果表明，自发性高血压的病理状态可影响EC活性成分在大鼠体内的组织分布。
Eucommiae Cortex (EC), a rare, nourishing medicinal herb that is native in China, has good effect in the treatment of hypertension. In this study, we compared tissue distribution of six representative active components of EC extract-genipinic acid (GA), protocatechuic acid (PCA), neochlorogenic acid (NCA), chlorogenic acid (CA), (+)-pinoresinol di-O-beta-D-glucopyranoside (PDG), and (+)-pinoresinol 4 '-O-beta-D-glucopyranoside (PG)-between normal rats and spontaneously hypertensive rats (SHRs). Each rat was intragastrically given EC extract at a dose of 5.4 g/kg. Rats were sacrificed at 10 min, 30 min, 2 h, and 8 h after administration; six rats were sacrificed at each time point. Then, we quickly harvested their major organs, including heart, liver, spleen, lungs, kidneys, and brain. Using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), we determined the levels of the above mentioned six components in the organs of both types of rats and then analyzed differences in the tissue distribution. The results showed that levels of each component differed between SHRs and the normal group at each time point. As time progressed, the number of organs in which GA distribution in each tissue of SHRs differed from that of the normal group gradually increased: SHRs showed lower GA levels than normal rats. Levels of PG and PDG in both groups at 10 and 30 min were similar. NCA and CA in the SHR group and the normal group at 10 min, 30 min, and 2 h were also similar to some extent. The results indicated that the pathological state of spontaneous hypertension could affect tissue distribution of EC active components in rats.