Exosomal Circular RNA as a Biomarker Platform for the Early Diagnosis of Immune-Mediated Demyelinating Disease
Exosomal Circular RNA as a Biomarker Platform for the Early Diagnosis of Immune-Mediated Demyelinating Disease
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DOI：
10.3389/fgene.2019.00860
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发表时间：
2019-09-27
影响因子：
3.7
通讯作者：
Yang, Qiwei
中科院分区：
文献类型：
--
作者：
He, Jinting;Ren, Ming;Yang, Qiwei
外泌体可以穿过血脑屏障并存在于脑脊液（CSF）中。外泌体中的DNA、RNA、蛋白质和脂质等成分变化很大，与疾病进展密切相关。环状RNA（circRNA）结构稳定，在外泌体中具有较长的半衰期而不会降解。因此，circRNA被认为是一种理想的生物标志物，可用于监测多种中枢神经系统疾病。本研究旨在研究免疫介导的脱髓鞘疾病患者CSF中exosomal circRNA（exo-circRNA）的表达谱，以确定合适的生物标志物用于免疫介导的脱髓鞘疾病的早期诊断。使用杂交阵列分析从来自免疫介导的脱髓鞘疾病患者的五个CSF样品和五个配对的CSF对照样品获得的外来体中的circRNA表达水平。分层聚类分析显示，5，095个exo-circRNA在免疫介导的脱髓鞘疾病患者和配对对照样品之间差异表达。在这些exo-circRNA中，有26种被鉴定为在免疫介导的脱髓鞘疾病患者的CSF外泌体中显着差异表达（FC >= 1.5且p
Exosomes can pass through the blood-brain barrier and are present in the cerebrospinal fluid (CSF). The components in exosomes, such as DNA, RNA, protein, and lipids, change greatly and are closely related to disease progression. Circular RNA (circRNA) is stable in structure and has a long half-life in exosomes without degradation. Therefore, circRNA is considered an ideal biomarker and can be used to monitor a variety of central nervous system diseases. This study aimed to investigate the expression profiles of exosomal circRNA (exo-circRNA) in CSF from patients with immune-mediated demyelinating diseases to identify suitable biomarkers for the early diagnosis of immune-mediated demyelinating diseases. circRNA expression levels in exosomes obtained from five CSF samples from immune-mediated demyelinating disease patients and five paired CSF control samples were analyzed using a hybridization array. Hierarchical clustering analysis showed that 5,095 exo-circRNAs were differentially expressed between patients with immune-mediated demyelinating diseases and paired control samples. Of these exo-circRNAs, 26 were identified as significantly differentially expressed in CSF exosomes from patients with immune-mediated demyelinating diseases (FC >= 1.5 and p