γ-tocopherol supplementation alone and in combination with α-tocopherol alters biomarkers of oxidative stress and inflammation in subjects with metabolic syndrome
γ-tocopherol supplementation alone and in combination with α-tocopherol alters biomarkers of oxidative stress and inflammation in subjects with metabolic syndrome
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DOI：
10.1016/j.freeradbiomed.2007.12.018
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发表时间：
2008-03-15
影响因子：
7.4
通讯作者：
Jialal, Ishwarlal
中科院分区：
文献类型：
--
作者：
Devaraj, Sridevi;Leonard, Scott;Jialal, Ishwarlal
代谢综合征（MetS）与糖尿病和心血管疾病（CVD）的发病率增加有关。α-生育酚（AT）的前瞻性临床试验尚未产生阳性结果。因为AT补充降低了循环γ-生育酚（GT），我们评估了单独补充GT（800 mg/天）、AT（800 mg/天）、组合或安慰剂6周的MetS受试者中的AT和GT浓度、氧化应激的生物标志物和炎症（n = 20/组）。血浆AT和GT水平增加后补充AT单独或GT单独或组合。AT补充显著降低GT水平。尿液中的α-和γ-CEHC（各自T的代谢物）也相应增加，即，与安慰剂相比，α-CEHC与AT和γ-CEHC与GT补充。AT+GT联合组HsCRP水平显著降低。LPS激活的全血释放IL-1和IL-6没有变化。AT单独或与GT联合应用可显著降低TNF水平。AT、GT或联合应用可显著降低血浆MDA/HNE和脂质过氧化物水平。与安慰剂相比，硝基酪氨酸水平仅用GT或GT+AT显著降低，而用AT不显著降低。因此，AT和GT补充的组合似乎在氧化应激和炎症的生物标志物上上级单独的任一补充，并且需要在前瞻性临床试验中进行测试以阐明其在CVD预防中的效用。(C)2007年爱思唯尔公司All rights reserved.
Metabolic syndrome (MetS) is associated with increased incidence of diabetes and cardiovascular disease (CVD). Prospective clinical trials with alpha-tocopherol (AT) have not yielded positive results. Because AT supplementation decreases circulating gamma-tocopherol (GT), we evaluated supplementation with GT (800 mg/day), AT (800 mg/day), the combination or placebo for 6 weeks alone AT and GT concentrations, biomarkers of oxidative stress, and inflammation in subjects with MetS (n = 20/group). Plasma AT and GT levels increased following supplementation with AT alone or GT alone or in combination. AT supplementation significantly decreased GT levels. Urinary alpha- and gamma-CEHC, metabolites of the respective Ts, also increased correspondingly, i.e., alpha-CEHC with AT and gamma-CEHC with GT supplementation, compared to placebo. HsCRP levels significantly decreased in the combined AT+GT group. LPS-activated whole blood release of IL-1 and IL-6 did not change. There was a significant decrease in TNF with AT alone or in combination with GT. Plasma MDA/HNE and lipid peroxides were significantly decreased with AT, GT, or in combination. Nitrotyrosine levels were significantly decreased only with GT or GT+AT but not with AT compared to placebo, Thus, the combination of AT and GT supplementation appears to be superior to either supplementation alone on biomarkers of oxidative stress and inflammation and needs to be tested in prospective clinical trials to elucidate its utility in CVD prevention. (C) 2007 Elsevier Inc. All rights reserved.