The contribution of surface waxes to pre-penetration growth of an entomopathogenic fungus on host cuticle
The contribution of surface waxes to pre-penetration growth of an entomopathogenic fungus on host cuticle
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DOI：
10.1016/j.mycres.2006.10.007
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发表时间：
2007-02-01
影响因子：
--
通讯作者：
Charnley, A. Keith
中科院分区：
文献类型：
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作者：
Jarrold, Samantha L.;Moore, David;Charnley, A. Keith
利用蝗虫翅生物测定法，在穿透前将昆虫病原真菌从寄主角质层中去除，研究了表面脂类和蜡质在特定蝗虫病原菌绿僵菌穿透前生长中的作用。无花果。扫描电子显微镜和原子力电子显微镜显示了真菌对角质层表面结构的影响。尽管真菌可以在真正的烷烃上萌发，作为唯一的碳源，但只有低水平的萌发发生在含有长链正构烷烃和其他蜡质(特别是通过气相色谱和质谱学鉴定)的非极性翅膀角质层粗提物上。在翅膀上的预渗透生长过程中，真菌去除了很大比例的非极性和极性成分。中国血竭的极地粗提物含有脂肪酸、脂肪酸酯、葡萄糖、氨基酸和多肽，对萌发有很强的促进作用，而提取的刺槐后翅发芽较差。因此，在真菌利用复杂的非极性脂类混合物之前，可能需要同样存在于真菌表面的简单极性化合物来刺激萌发。(C)2006年英国真菌学会。爱思唯尔有限公司出版。保留所有权利。
A locust wing bioassay, that allowed an entomopathogenic fungus to be removed from host cuticle before penetration, was used to investigate the role of surface lipids and waxes in pre-penetration growth of the specific locust pathogen Metarhizium anisopliae var. acridum. SEM and atomic force electron microscopy showed the impact of the fungus on the architecture of the cuticle surface. Although the fungus can germinate on authentic alkanes as the sole carbon source, only low levels of germination occurred on crude, non-polar wing cuticle extracts, containing a mixture of long-chain n-alkanes and other waxes (identified in particular by gas chromatography and mass spectroscopy). The fungus removed a large proportion of non-polar and polar components during pre-penetration growth on the wing. Polar crude extracts from Schistocerca gregaria hindwings, which contained fatty acids, fatty acid esters, glucose, amino acids and peptides, were strong promoters of germination, and poor germination was observed on a locust hindwing from which the extract had been taken. Thus simple polar compounds, also present on the surface, may be required to stimulate germination before the fungus can make use of a complex mixture of non-polar lipids. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.