Molecular Phylogeny of the Papilionoideae (Family Leguminosae): RbcL Gene Sequences versus Chemical Taxonomy
Molecular Phylogeny of the Papilionoideae (Family Leguminosae): RbcL Gene Sequences versus Chemical Taxonomy
复制标题
蝶形花亚科（豆科）的分子系统发育：RbcL 基因序列与化学分类学
DOI：
10.1111/j.1438-8677.1995.tb00845.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Wink
中科院分区：
文献类型：
--
作者：
E. Käss;M. Wink
从豆科蝶形花科蝶形蝶亚科蝶亚科蝶形蝶亚科蝶形蝶亚科蝶亚科蝶采用聚合酶链反应（PCR）扩增rbcL基因，直接测序。RbcL序列进行了评估与字符状态（最大简约法; PAUP）和距离的方法（相邻连接; MEGA）。基于形态学的族和亚族分类大多与rbcL发生一致。槐属的成员和Genista/Cytisus复合体内的部落内关系存在差异：槐属出现在两个分支中，一个分支与S。japonica在Papilionoideae的基础上和第二个更先进的组（与S。davidii，刺毛藓S. jaubertii和S.flavescens），其代表Thermopsideae的姐妹分支。金雀花属复合体包括金雀花属（Cytisus）、雀花属（Chamaacutisus）、杯花属（Calicotome）和密花属（Spartocytisus），但不包括金雀花属（Cytisophyllum），后者是染料木属复合体的一个独特成员。Chamaespartium sagittale是非常接近Genista支持的观点，它不代表一个独立的属，但应被视为Genista sagittalis。与染料木属相近的是Teline和Spartium，而Argyrolobium、Retama、Cytisophyllum、Ulex、Petteria、Adenocarpus、Chamaespartium tridentatum和Laburnum可以被认为是严格意义上的染料木属的“离群值”。RbcL的合成与生物碱和其他天然产物的合成进行了比较。第一个结果表明，次级代谢产物的配置文件，如果与形态或rbcL序列相比，作为一个分类标记的价值有限。
Total DNA was extracted from 67 species (30 genera) of the subfamily Papilionoideae (family Leguminosae). The rbcL gene was amplified by polymerase chain reaction (PCR) and sequenced directly. RbcL sequences were evaluated with character-state (maximum parsimony; PAUP) and distance methods (neighbour-joining; MEGA). Morphology-based classifications of tribes and subtribes are mostly congruent with rbcL phylogeny. Differences occur for members of the genus Sophora and for intratribal relationships within the Genista/Cytisus complex: Sophora appears in two clades; one clade with S. japonica at the base of the Papilionoideae and a second more advanced group (with S. davidii, S. jaubertii, and S.flavescens) which represents a sister clade of the Thermopsideae. The Cytisus complex includes the genera Cytisus, Chamaecytisus, Calicotome and Spartocytisus but not Cytisophyllum which appears as a distinct member of the Genista complex. Chamaespartium sagittale is very close to Genista supporting the view that it does not represent an independent genus but should be treated as Genista sagittalis. Close to Genista are Teline and Spartium, whereas Argyrolobium, Retama, Cytisophyllum, Ulex, Petteria, Adenocarpus, Chamaespartium tridentatum and Laburnum can be considered as “outliers” of the Genista-group sensu stricto. RbcL phylogeny is compared with profiles of alkaloids and other natural products. First results indicate that secondary metabolite profiles, if compared with morphology or rbcL sequences, are of limited value as a taxonomic marker.