The stem of Sinomenium acutum inhibits mast cell-mediated anaphylactic reactions and tumor necrosis factor-α production from rat peritoneal mast cells
The stem of Sinomenium acutum inhibits mast cell-mediated anaphylactic reactions and tumor necrosis factor-α production from rat peritoneal mast cells
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DOI：
10.1016/s0378-8741(99)00160-9
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Lee, EJ
中科院分区：
文献类型：
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作者：
Kim, HM;Moon, PD;Lee, EJ
青藤水提取物（SSAE）（0.1-1000 mg/kg）剂量依赖性地抑制化合物48/80引起的小鼠全身过敏反应。特别地，SSAE在1000mg/kg的剂量下将化合物48/80诱导的过敏反应降低了50％。 SSAE (100-1000 mg/kg) 还显着抑制抗二硝基苯基 (DNP) IgE 激活的局部过敏反应。当小鼠接受0.1至1000 mg/kg浓度的SSAE预处理时，血浆组胺水平以剂量依赖性方式降低。 SSAE (1-1000 μg/ml) 剂量依赖性地抑制由化合物 48/80 或抗 DNP IgE 激活的大鼠腹膜肥大细胞 (RPMC) 释放组胺。此外，SSAE（0.1μg/ml）对抗DNP IgE诱导的肿瘤坏死因子-α（TNF-α）产生具有显着的抑制作用。这些结果表明 SSAE 抑制肥大细胞介导的过敏反应和肥大细胞产生 TNF-α。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
The aqueous extract of Sinomenium acutum stem (SSAE) (0.1-1000 mg/kg) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. In particular, SSAE reduced compound 48/80-induced anaphylactic reaction with 50% at the dose of 1000 mg/kg. SSAE (100-1000 mg/kg) also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with SSAE at a concentration ranging from 0.1 to 1000 mg/kg, the plasma histamine levels were reduced in a dose-dependent manner. SSAE (1-1000 mu g/ml) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMCs) activated by compound 48/80 or anti-DNP IgE. In addition, SSAE (0.1 mu g/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha (TNF-alpha) production. These results indicate that SSAE inhibits mast cell-mediated anaphylactic reactions and TNF-alpha production from mast cells. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.