Developmental succession of the microbiome of Culex mosquitoes.
Developmental succession of the microbiome of Culex mosquitoes.
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DOI：
10.1186/s12866-015-0475-8
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发表时间：
2015-07-24
期刊：
影响因子：
4.2
通讯作者：
Walton WE
中科院分区：
文献类型：
--
作者：
Duguma D;Hall MW;Rugman-Jones P;Stouthamer R;Terenius O;Neufeld JD;Walton WE
与蚊子相关的本地微生物区系在蚊子的发育和媒介能力中发挥着重要作用。对库蚊整个发育阶段（早期和晚期幼虫龄、蛹和成虫）16S rRNA 基因的细菌 V3 区域进行测序，用于检验库蚊幼虫阶段发现的细菌跨体传播到成虫阶段的假设，并比较现场收集的蚊子与实验室饲养的蚊子的微生物组。 Beta多样性分析表明，跗库蚊三个生命阶段（幼虫、蛹和成虫）的细菌群落结构存在差异。尽管在所有阶段中只发现了细菌 OTU 总数的约 2%，但这些 OTU 的序列占从所有阶段恢复的细菌序列总数的近 82%。 Thorsellia（Gammaproteobacteria）是在野外收集的库蚊的所有发育阶段中发现的最丰富的细菌分类群，但在实验室饲养的蚊子群落的蚊子中很少见。 Thorsellia 序列在蚊子生命阶段的微生物组中的比例在个体发育上有所不同，从 C. tarsalis 的蛹中恢复的比例最大，而从新出现的成虫中恢复的比例最低。由于栖息地年龄或生物农药处理而导致的栖息地微生物群差异并没有显着影响现场收集的晚龄幼虫的微生物群。早期幼虫的微生物组多样性最高，而实验室饲养的蚊子的微生物组多样性最低。与晚龄幼虫、蛹和新羽化成虫相比，早龄跗螈幼虫的细菌群落明显更加多样化。在早期龄幼虫中发现的一些细菌 OTU 也存在于整个发育阶段。 Thorsellia 在现场收集的未成熟阶段的细菌群落中占主导地位，但在成虫中的相对丰度要低得多。在幼虫栖息地中观察到的微生物群差异并不影响晚龄幼虫或成虫的细菌群落特征。然而，实验室饲养的跗螈幼虫中的细菌群落与野外存在显着差异。确定托尔塞利亚在蚊子中的作用及其在不同种类蚊子中的分布值得进一步研究。本文的在线版本 (doi:10.1186/s12866-015-0475-8) 包含补充材料，可供授权用户使用。
The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that bacteria found in the larval stage of Culex are transstadially transmitted to the adult stage, and to compare the microbiomes of field-collected versus laboratory-reared mosquitoes. Beta diversity analysis revealed that bacterial community structure differed among three life stages (larvae, pupae and adults) of Culex tarsalis. Although only ~2 % of the total number of bacterial OTUs were found in all stages, sequences from these OTUs accounted for nearly 82 % of the total bacterial sequences recovered from all stages. Thorsellia (Gammaproteobacteria) was the most abundant bacterial taxon found across all developmental stages of field-collected Culex mosquitoes, but was rare in mosquitoes from laboratory-reared colonies. The proportion of Thorsellia sequences in the microbiomes of mosquito life stages varied ontogenetically with the greatest proportions recovered from the pupae of C. tarsalis and the lowest from newly emerged adults. The microbiome of field-collected late instar larvae was not influenced significantly by differences in the microbiota of the habitat due to habitat age or biopesticide treatments. The microbiome diversity was the greatest in the early instar larvae and the lowest in laboratory-reared mosquitoes. Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults. Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages. Thorsellia dominated the bacterial communities in field-collected immature stages but occurred at much lower relative abundance in adults. Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults. However, bacterial communities in laboratory-reared C. tarsalis larvae differed significantly from the field. Determining the role of Thorsellia in mosquitoes and its distribution across different species of mosquitoes warrants further investigation. The online version of this article (doi:10.1186/s12866-015-0475-8) contains supplementary material, which is available to authorized users.