Purification and characterization of Eikenella corrodens type IV pilin.
Purification and characterization of Eikenella corrodens type IV pilin.
复制标题
IV 型菌毛艾肯氏菌的纯化和表征。
DOI：
10.1128/iai.63.9.3693-3696.1995
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发表时间：
1995
影响因子：
3.1
通讯作者：
Hirschberg,R
中科院分区：
文献类型：
--
作者：
Hood,BL;Hirschberg,R
腐蚀艾肯氏菌是一种与牙周病和软组织感染相关的革兰氏阴性人类病原体。采用结合-解离法和快速蛋白液相色谱法纯化Pilin；它的表观分子质量约为14.8 kDa， n端氨基酸序列反映IV型柱蛋白。纯化蛋白的抗体与整个细胞上的菌毛反应。这是首次报道从该菌中纯化IV型菌毛/菌毛蛋白。其他IV型菌毛是重要的毒力因子；我们目前正在研究毛在锈菌中的生物学作用。
Eikenella corrodens is a gram-negative human pathogen associated with periodontal diseases and soft-tissue infections. Pilin was purified by association-dissociation and fast protein liquid chromatography; it had an apparent molecular mass of about 14.8 kDa and an N-terminal amino acid sequence reflective of type IV pilins. Antibodies to the purified protein reacted with pili on whole cells. This is the first report of purification of type IV pili/pilin from this organism. Other type IV pili are important virulence factors; we are currently investigating the biological role of pili in E. corrodens.