Comparison of the structural and kinetic properties of the cytochrome c nitrite reductases from Escherichia coli, Wolinella succinogenes, Sulfurospirillum deleyianum and Desulfolvibrio desulfuricans
Comparison of the structural and kinetic properties of the cytochrome c nitrite reductases from Escherichia coli, Wolinella succinogenes, Sulfurospirillum deleyianum and Desulfolvibrio desulfuricans
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DOI：
10.1042/bst0340143
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发表时间：
2006-02-01
影响因子：
3.9
通讯作者：
Richardson, DJ
中科院分区：
文献类型：
--
作者：
Clarke, TA;Hemmings, AM;Richardson, DJ
最近从硫代螺杆菌、deleyi anum、产生琥珀酸狼疮杆菌、大肠杆菌和硫化硫弧菌中分离出的NrfA的结晶学特征使我们能够识别出结构保守的区域。不同细菌亚硝酸盐和亚硫酸盐还原酶活性的比较表明，不同生物体的相对活性有所不同。通过氨基酸序列和结构的比较，可以发现单体-单体界面和活性中心通道的差异；这些差异可能是底物活性差异的原因，并表明NRFA结构的细微变化可能会优化酶的不同作用。
The recent crystallographic characterization of NrfAs from Sulfurospirillum, deleyianum, Wolinella succinogenes, Escherichia coli and Desulfovibrio desulfuricans allows structurally conserved regions to be identified. Comparison of nitrite and sulphite reductase activities from different bacteria shows that the relative activities vary according to organism. By comparison of both amino acid sequences and structures, differences can be identified in the monomer-monomer interface and the active-site channel; these differences could be responsible for the observed variance in substrate activity and indicate that subtle changes in the NrfA structure may optimize the enzyme for different roles.