PGMA-based supramolecular hyperbranched polycations for gene delivery
PGMA-based supramolecular hyperbranched polycations for gene delivery
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DOI：
10.1039/c6py00759g
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发表时间：
2016-01-01
影响因子：
4.6
通讯作者：
Xu, Fu-Jian
中科院分区：
文献类型：
--
作者：
Qi, Miao;Duan, Shun;Xu, Fu-Jian
超分子化学在生物医学领域有着广泛的应用。研究表明，分子的拓扑结构对基因传递系统的性能有重要影响。具有超支化拓扑结构的类超分子聚合物基因载体尚未见报道。本工作基于原子转移自由基聚合，合成了一系列乙醇胺功能化的聚甲基丙烯酸缩水甘油酯（PGMA）臂AB 2型大分子单体。AB 2型大分子单体通过金刚烷与β-环糊精之间的主客体相互作用可以自组装成超分子超支化聚合物。详细评价了自组装和自组装的超支化聚阳离子的生物物理性质。自组装的具有超支化结构的超分子聚阳离子具有更高的pDNA缩合能力和更好的基因转染性能。本工作为设计高性能的超分子超支化核酸递送系统提供了新的途径。
Supramolecular chemistry has been widely applied in biomedical fields. It was reported that the topological structure has effects on the performance of gene delivery systems. Supramolecular polymer-like gene carriers with hyperbranched topological structures have still not been reported. In this work, a series of ethanolamine-functionalized polyglycidyl methacrylate (PGMA)-armed AB2 type macromonomers were synthesized based on atom transfer radical polymerization. The AB2 type macromonomers can self-assemble into supramolecular hyperbranched polymers by the host–guest interaction between adamantane and β-cyclodextrin. The biophysical properties of self-assembled and dissembled supramolecular hyperbranched polycations were evaluated in detail. The self-assembled supramolecular polycations with hyperbranched structures possessed higher pDNA condensation ability and much better gene transfection performances than the dissembled AB2 type counterparts. The present work would provide a new venue for designing advanced high-performance supramolecular hyperbranched nucleic acid-delivery systems.