Low Endotoxic Potential of Legionella pneumophilaLipopolysaccharide due to Failure of Interaction with the Monocyte Lipopolysaccharide Receptor CD14
Low Endotoxic Potential of Legionella pneumophilaLipopolysaccharide due to Failure of Interaction with the Monocyte Lipopolysaccharide Receptor CD14
复制标题
由于与单核细胞脂多糖受体 CD14 相互作用失败导致嗜肺军团菌脂多糖的低内毒性潜力
作者：
B. Neumeister;M. Faigle;M. Sommer;U. Zähringer;F. Stelter;R. Menzel;C. Schütt;H. Northoff
摘要嗜肺军团菌是一种引起军团病和庞蒂亚克热的革兰氏阴性细菌，体外凝胶法显示该菌具有较强的凝胶性，但在兔和小鼠体内不能引起发热和局部Shwartzman反应。我们分析了纯化的嗜肺乳杆菌脂多糖(LPS-LP)诱导人单核细胞株Mono Mac 6活化的能力，其表现为促炎症细胞因子的分泌和对随后的LPS刺激的脱敏。我们的研究表明，尽管细菌内毒素的反应性正常，但在Mono Mac 6细胞中诱导细胞因子的分泌和对内毒素攻击的脱敏所需的细菌内毒素浓度是明尼苏达州肠炎沙门氏菌的1000倍。因此，我们研究了内毒素-LP与内毒素受体CD14的相互作用。结果表明，脂多糖-LP不与CHO细胞表达的膜结合CD14结合，也不与可溶性CD14反应。我们的结果提示，低内毒素潜能是由于与内毒素受体CD14相互作用失败所致。
ABSTRACT Legionella pneumophila, a gram-negative bacterium causing Legionnaires’ disease and Pontiac fever, was shown to be highly reactive in in vitro gelation of Limulus lysate but not able to induce fever and the local Shwartzman reaction in rabbits and mice. We analyzed the capacity of purified L. pneumophila lipopolysaccharide (LPS-Lp) to induce activation of the human monocytic cell line Mono Mac 6, as revealed by secretion of proinflammatory cytokines and desensitization to subsequent LPS stimulation. We showed that despite normal reactivity of LPS-Lp in theLimulus amoebocyte lysate assay, induction of cytokine secretion in Mono Mac 6 cells and desensitization to an endotoxin challenge required LPS-Lp concentrations 1,000 times higher than for LPS of Salmonella enterica serovar Minnesota. Therefore, we examined the interaction of LPS-Lp with the LPS receptor CD14. We demonstrated that LPS-Lp did not bind to membrane-bound CD14 expressed on transfected CHO cells, nor did it react with soluble CD14. Our results suggest that the low endotoxic potential of LPS-Lp is due to a failure of interaction with the LPS receptor CD14.
影响因子：
4.4
作者：
M. Luchi;R. Munford
通讯作者：
M. Luchi;R. Munford
CD14 结合脂多糖的分析。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kirkland,TN;Finley,F;Leturcq,D;Moriarty,A;Lee,JD;Ulevitch,RJ;Tobias,PS
通讯作者：
Tobias,PS