SYSTEMIC ADMINISTRATION OF LEPTIN POTENTIATES THE RESPONSE OF NEURONS IN THE NUCLEUS OF THE SOLITARY TRACT TO CHEMORECEPTOR ACTIVATION IN THE RAT
SYSTEMIC ADMINISTRATION OF LEPTIN POTENTIATES THE RESPONSE OF NEURONS IN THE NUCLEUS OF THE SOLITARY TRACT TO CHEMORECEPTOR ACTIVATION IN THE RAT
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DOI：
10.1016/j.neuroscience.2012.10.065
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发表时间：
2013-01-15
期刊：
影响因子：
3.3
通讯作者：
Moreau, J. M.
中科院分区：
文献类型：
--
作者：
Ciriello, J.;Moreau, J. M.
孤束核（NTS）内微量注射瘦素可引起交感神经兴奋性反应，并增强心血管对化学反射激活的反应。在这项研究中，实验进行了Sprague道利大鼠最初提供了一个详细的映射内的NTS复合物的细胞含有免疫反应性的长形式的瘦素受体（Ob-Rb）。在第二个系列中，这NTS地区含有OB-Rb免疫反应细胞探索单一单位逆向激活的升压网站在头端延髓腹外侧（RVLM）的刺激。然后测试这些逆向鉴定的神经元对颈动脉内注射瘦素（50-100 ng/0.1 ml）的反应，以及对颈动脉内注射氰化钾（KCN）（80 μ g/0.1 ml）后外周化学感受器激活的反应。含有OB-Rb样免疫反应的细胞主要发现在尾部NTS：在内侧，连合和胶状（亚postremal区）的NTS复杂的亚核。在这些NTS区域中测试的73个单单位中，48个通过刺激RVLM升压部位逆向激活，其中25个单单位在颈动脉内注射瘦素后放电率增加。此外，这些瘦素反应神经元中的17个被颈动脉内注射KCN（80 μ/0.1 ml）兴奋。此外，这些单一单位的兴奋性反应KCN增强（59-83%）后立即注射瘦素。这些数据表明，瘦素反应神经元在NTS介导化学感受器传入的信息，加压网站的RVLM，并建议瘦素可能作为一个促进剂的NTS内的神经元回路，加强交感神经兴奋反应引起的反射激活动脉化学感受器。(C)2012年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Leptin microinjections into the nucleus of the solitary tract (NTS) have been shown to elicit sympathoexcitatory responses, and potentiate the cardiovascular responses to activation of the chemoreflex. In this study, experiments were done in Sprague Dawley rats initially to provide a detailed mapping within the NTS complex of cells containing immunoreactivity to the long form of the leptin receptor (Ob-Rb). In a second series, this NTS region containing Ob-Rb immunoreactive cells was explored for single units antidromically activated by stimulation of pressor sites in the rostral ventrolateral medulla (RVLM). These antidromically identified neurons were then tested for their response to intra-carotid injections of leptin (50-100 ng/0.1ml), and to activation of peripheral chemoreceptors following an injection of potassium cyanide (KCN) (80 mu g/0.1ml) into the carotid artery. Cells containing Ob-Rb-like immunoreactivity were found predominantly in the caudal NTS: within the medial, commissural and gelatinous (sub-postremal area) subnuclei of the NTS complex. Of 73 single units tested in these NTS regions, 48 were antidromically activated by stimulation of RVLM pressor sites and 25 of these single units responded with an increase in discharge rate after intra-carotid injections of leptin. In addition, 17 of these leptin responsive neurons were excited by the intra-carotid injections of KCN (80 mu/0.1 ml). Furthermore, the excitatory response of these single units to KCN was potentiated (59-83%) immediately following the leptin injection. These data indicate that leptin responsive neurons in NTS mediate chemoreceptor afferent information to pressor sites in the RVLM, and suggest that leptin may act as a facilitator on neuronal circuits within the NTS that potentiates the sympathoexcitatory responses elicited during the reflex activation of arterial chemoreceptors. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.