RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS
RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS
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DOI：
10.1016/0022-1759(83)90303-4
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
MOSMANN, T
中科院分区：
文献类型：
--
作者：
MOSMANN, T
用四唑盐建立了哺乳动物细胞存活和增殖的定量比色测定法。该测定检测活细胞，但不检测死细胞，并且产生的信号取决于细胞的活化程度。因此，该方法可用于测量细胞毒性、增殖或活化。结果可以在多孔扫描分光光度计上读取，并显示出高精度。试验中未使用洗涤步骤。比色法的主要优点是快速、准确，而且不需要任何放射性同位素。该试验用于测量增殖性淋巴因子、有丝分裂原刺激和补体介导的溶解。
A tetrazolium salt was used develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer and show a high degree of precision. No washing steps are used in the assay. The main advantages of the colorimetric assay are its rapidity and precision, and the lack of any radioisotope. The assay was used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.