CFTR mRNA and its truncated splice variant (TRN-CFTR) are differentially expressed during collecting duct ontogeny
CFTR mRNA and its truncated splice variant (TRN-CFTR) are differentially expressed during collecting duct ontogeny
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DOI：
10.1016/s0014-5793(98)00112-4
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发表时间：
1998-02-27
期刊：
影响因子：
3.5
通讯作者：
Horster, M
中科院分区：
文献类型：
--
作者：
Huber, S;Braun, G;Horster, M
集合管上皮起源于胚胎输尿管的分支形态发生。采用定量逆转录-聚合酶链反应（RT-PCR）技术，在大鼠输尿管芽和皮质集合管的原代单层培养中，对CFTR mRNA表达的个体发育依赖性变化进行了评估。在胚胎妊娠第E15天和出生后第P1天之间，UB中野生型CFTR特异性PCR产物的量下降至初始值的20%。出生后，CFTR产物在P1和P7之间短暂增加10倍，并在P14天下降。然而，TRN-CFTR（一种截短的剪接变体）特异性的PCR产物在早期胚胎细胞中较低，在E17和P2天之间显著增加，并在出生后达到平台期。因此，编码TRN-CFTR的mRNA似乎不具有特定的胚胎形态发生功能。相比之下，野生型CFTR mRNA的这种功能，因为它的丰度是高的早期胚胎肾，以及在出生后的时期前不久分支形态建成完成。(C)1998年欧洲生物化学学会联合会。
The collecting duct epithelium originates from the embryonic ureter by branching morphogenesis. Ontogeny-dependent changes of CFTR mRNA expression were assessed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in primary monolayer cultures of rat ureteric buds CUB) and cortical collecting ducts, microdissected at different embryonic and postnatal developmental stages. The amount of wild-type CFTR-specific PCR product in UB declined to 20% of the initial value between embryonic gestational day E15 and postnatal day P1. After birth the CFTR product increased transiently between P1 and P7 by a factor of 10 and decreased towards day P14. PCR products specific for TRN-CFTR, a truncated splice variant, however, were low in early embryonic cells, increased markedly between day E17 and P2, and reached a plateau postnatally. Therefore, mRNA encoding TRN-CFTR does not appear to have a specific embryonic-morphogenetic function. By contrast, such function is suggested for wild-type CFTR mRNA as its abundance was high in early embryonic nephrogenesis, as well as during a postnatal period shortly before branching morphogenesis is completed. (C) 1998 Federation of European Biochemical Societies.