Evolution of wheat gliadins conformation during film formation:: A Fourier transform infrared study
Evolution of wheat gliadins conformation during film formation:: A Fourier transform infrared study
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DOI：
10.1021/jf0009899
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发表时间：
2001-02-01
影响因子：
6.1
通讯作者：
Guéguen, J
中科院分区：
文献类型：
--
作者：
Mangavel, C;Barbot, J;Guéguen, J
通过傅立叶变换红外光谱法研究了膜形成溶液中小麦麦芽胶蛋白（与面筋的主要储存蛋白分数）的二级结构及其在膜形成过程中的演变。在膜形成的溶液中，小麦麦粒胶质蛋白呈现出不同的二级结构的混合物，并具有由脯氨酸残基诱导的β-转化的重要贡献。增塑剂的存在对膜形成溶液中的蛋白质二级结构没有任何影响。在干燥过程中遵循蛋白质构象的演变；这种进化的主要特征是，红外带的清晰生长在1622 cm（-1），这是分子间氢键β-β-β-键β的特征。这揭示了膜干燥过程中蛋白质聚集体的形成。还研究了干燥温度对膜特性和麦醇溶蛋白二级结构的影响。较高的干燥温度引起了膜的拉伸强度和β-元素骨料的量的增加。尽管已经描述了热诱导的二硫键交联的出现，但有明确的证据表明，氢键β-β-β-元素骨料聚集体也通过热处理诱导。但是，不可能确定这些聚集体的发生与膜的拉伸强度的增加之间是否存在直接关系。
The secondary structures of wheat gliadins (a major storage protein fraction from gluten) in film-forming solutions and their evolution during film formation were investigated by Fourier transform infrared spectroscopy. In the film-forming solution, wheat gliadins presented a mixture of different secondary structures, with an important contribution of beta -turns induced by proline residues. The presence of plasticizer did not have any influence on protein secondary structure in the film-forming solution. The evolution of protein conformation was followed during drying; the major feature of this evolution was a clear growing of the infrared band at 1622 cm(-1), characteristic of intermolecular hydrogen-bonded beta -sheets. This revealed the formation of protein aggregates during film drying. The influence of the drying temperature on film properties and gliadin secondary structures was also investigated. Higher drying temperatures induced an increase of both the tensile strength of the films and the amount of beta -sheets aggregates. Although the appearance of heat-induced disulfide bridge cross-links has already been described, there is clear evidence that hydrogen-bonded beta -sheets aggregates are also induced by thermal treatment. It was not possible, however, to determine whether there is a direct relationship between the occurrence of these aggregates and the increase of the tensile strength of the films.