PLASTICITY OF POSTGANGLIONIC SYMPATHETIC NEURONS IN THE RAT SUPERIOR CERVICAL-GANGLION AFTER AXOTOMY
PLASTICITY OF POSTGANGLIONIC SYMPATHETIC NEURONS IN THE RAT SUPERIOR CERVICAL-GANGLION AFTER AXOTOMY
复制标题
DOI：
10.1002/jemt.1070290209
复制
发表时间：
1994-10-01
影响因子：
2.5
通讯作者：
HEYM, C
中科院分区：
文献类型：
--
作者：
KLIMASCHEWSKI, L;TRAN, TD;HEYM, C
轴突横断后，脊髓和迷走神经感觉神经元以及颅运动神经元中的神经肽galanin （GAL）和血管活性肠多肽（VIP）上调。在本研究中，双标记免疫荧光显示，这两种肽在大鼠交感颈上神经节的主要神经节神经元（PGN）中增加。与含有不超过1% GAL或vip阳性细胞的对照神经节相比，约26%的PGN在节后主要分支横断后第1天表现出GAL免疫反应性。免疫反应神经元比例在30天后达到最大值（40%），在切开后第二个月内下降到27%左右。VIP阳性神经元的比例远低于GAL: 2%的PGN在第1天表现出VIP免疫反应，约7%的PGN在30和60天后被观察到。为了进一步表征新GAL-和vip阳性PGN，在轴切术后12天测定它们的细胞直径。与平均总神经元直径24.8 μ m相比，GAL免疫反应神经元主要为小、中大小神经元（22.2 μ m），而VIP主要发生在较大的神经元（26.1 μ m）。除细胞体外，许多节内神经纤维染色为GAL或VIP阳性，特别是在第6天。最有可能的是，这些纤维代表轴突，正如MAP2（一种在神经元体细胞和树突中发现的细胞骨架蛋白）的缺失所表明的那样。预包埋免疫电镜显示，它们与神经节后核周建立直接的膜接触。有些细胞体和纤维含有两种多肽。GAL或VIP与酪氨酸羟化酶（酪氨酸羟化酶，儿茶酚胺合成的限速酶）共定位显示，在GAL或VIP阳性细胞中，TH的免疫反应性降低，反之亦然。然而，在30天和60天后，VIP或GAL和TH的染色强度没有差异。本文讨论了GAL和VIP对周围神经再生的可能影响及其受靶源性因子的调控。(C) 1994 Wiley-Liss, Inc。
The neuropeptides galanin (GAL) and vasoactive intestinal polypeptide (VIP) are upregulated in spinal and vagal sensory as well as in cranial motor neurons after axonal transection. In this study an increase of both peptides is demonstrated in axotomized principal ganglionic neurons (PGN) of the rat sympathetic superior cervical ganglion by use of double-labeling immunofluorescence. Compared to control ganglia that do not contain more than 1% GAL- or VIP-positive cells, about 26% of all PGN exhibit GAL immunoreactivity by day 1 after transection of the major postganglionic branches. The proportion of immunoreactive neurons reaches its maximum after 30 days (40%) and decreases to about 27% within the second month after axotomy. The percentage of VIP-positive neurons is much lower than for GAL: 2% of the PGN exhibit VIP immunoreactivity at day 1 and about 7% are observed 30 and 60 days after axotomy. In order to further characterize newly GAL- and VIP-positive PGN, their cell diameters were determined 12 days after axotomy. Compared to the mean overall neuron diameter of 24.8 mu m, GAL-immunoreactive neurons are predominantly of small and intermediate size (22.2 mu m), whereas VIP occurs mainly in larger neurons (26.1 mu m), Besides cell bodies, many intraganglionic nerve fibers stain positive for GAL or VIP, particularly at day 6. Most likely, these fibers represent axons, as indicated by the absence of MAP2, a cytoskeletal protein found in neuronal somata and dendrites. They establish direct membrane contacts with postganglionic perikarya, as revealed by pre-embedding immune-electron microscopy. Some cell bodies and fibers contain both peptides. Colocalization of GAL or VIP with tyrosine hydroxylase (TH), the rate-limiting enzyme of catecholamine synthesis, reveals a reduced immunoreactivity for TH in intensely GAL- or VIP-positive cells, and vice versa at day 6. However, no difference in staining intensity for VIP or GAL, and TH, is observed after 30 and 60 days. Possible implications of GAL and VIP for peripheral nerve regeneration and their regulation by target-derived factors are discussed. (C) 1994 Wiley-Liss, Inc.