Deinococcus reticulitermitis sp nov., isolated from a termite gut
Deinococcus reticulitermitis sp nov., isolated from a termite gut
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DOI：
10.1099/ijs.0.026567-0
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Liu, Shuang-Jiang
中科院分区：
文献类型：
--
作者：
Chen, Wen;Wang, Baojun;Liu, Shuang-Jiang
从中国白蚁（Reticulitermes chinensis Snyder）肠道中分离到菌株TM-1(T)。菌株TM-1(T)细胞呈革兰氏阴性，呈球形（直径1.0 ~ 2.0 μ m），不运动，不产孢，呈红色。菌株TM-1(T)对紫外线辐射具有抗性，在暴露于剂量为100 J m（-2）的紫外线后，存活率为34%。生长条件为20-40℃（最适30℃），pH 6.0-10.0（最适6.0-7.0），NaCl浓度为0- 1% (w/v)（最适0-0.4%）。基于16S rRNA基因序列的系统发育分析表明，菌株TM-1(T)与Deinococcus属成员有亲缘关系，序列相似性为87.0 ~ 94.0%。菌株TM-1(T)的肽聚糖中含有鸟氨酸、丙氨酸、甘氨酸和谷氨酸。菌株TM-1的细胞脂肪酸含量最高(T)为特征3 （C-16:1 omega 7c和/或C-16:1 omega 6c； 22.3%）和C-16:0（37.5%）。醌以MK-8为主。极性脂质谱包含三种糖磷脂、六种糖脂、一种氨基脂和三种未知脂质。该型菌株DNA中G + C含量为65.6 mol%。根据已有的系统发育、化学分类和表型数据，菌株TM-1(T)代表了Deinococcus属的一个新种，建议将其命名为Deinococcus reticulitermitis sp. nov.，以TM-1(T) (=CGMCC 1.10218（T)=NBRC 106334(T)）为型菌株。
Bacterial strain TM-1(T) was isolated from the gut of a wood-feeding termite, Reticulitermes chinensis Snyder. Cells of strain TM-1(T) were Gram-negative, spherical (1.0-2.0 mu m in diameter), non-motile, non-sporulating and red-pigmented. Strain TM-1(T) was resistant to UV radiation, showing 34% survival after exposure to UV light at a dose of 100 J m(-2). Growth occurred at 20-40 degrees C (optimum, 30 degrees C), at pH 6.0-10.0 (optimum, pH 6.0-7.0), and in the presence of 0-1 % (w/v) NaCl (optimum, 0-0.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TM-1(T) was related to members of the genus Deinococcus, with sequence similarities ranging from 87.0 to 94.0%. The peptidoglycan of strain TM-1(T) contained ornithine, alanine, glycine and glutamic acid. The most abundant cellular fatty acids of strain TM-1(T) were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c; 22.3%) and C-16:0 (37.5 %). MK-8 was the predominant quinone. The polar lipid profile contained three glycophospholipids, six glycolipids, one aminolipid and three unknown lipids. DNA of the type strain had a G + C content of 65.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain TM-1(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus reticulitermitis sp. nov. is proposed, with TM-1(T) (=CGMCC 1.10218(T)=NBRC 106334(T)) as the type strain.