Activity regulation of tyrosinase by using photoisomerizable inhibitors.
Activity regulation of tyrosinase by using photoisomerizable inhibitors.
复制标题
使用光致异构抑制剂调节酪氨酸酶的活性。
DOI：
10.1016/j.jbiotec.2003.10.010
复制
发表时间：
2004
影响因子：
4.1
通讯作者：
T. Tatsuma
中科院分区：
文献类型：
--
作者：
K. Komori;K. Yatagai;T. Tatsuma
酪氨酸酶的酶活性是基于抑制剂4-偶氮苯甲酸（ACA）和4,4'-偶氮苯二甲酸（ADCA）的顺反光异构化来控制的。就 ACA 而言，顺式形式比反式形式更能抑制酪氨酸酶催化的 L-酪氨酸氧化。相反，对于 ADCA，顺式形式的抑制作用较小。在反应过程中通过光照射可逆地控制氧化速率。在ACA存在下，紫外光照射使反式异构化为顺式，减缓了酪氨酸氧化，而可见光照射则向后异构化，加速了反应。相比之下，在 ADCA 存在的情况下，紫外线加速反应，可见光减缓反应。
Enzymatic activity of tyrosinase was controlled on the basis of cis–trans photoisomerization of inhibitors, 4-azobenzene carboxylic acid (ACA) and 4,4′-azobenzene dicarboxylic acid (ADCA). In the case of ACA, the cis form inhibited tyrosinase-catalyzed oxidation of l-tyrosine more strongly than the trans form. On the contrary, in the case of ADCA, the cis form was less inhibitory. The oxidation rate was controlled reversibly by light irradiation in the course of the reaction. In the presence of ACA, UV light irradiation, which isomerized trans to cis form, decelerated the tyrosine oxidation, while visible light irradiation, which isomerized backward, accelerated the reaction. In contrast, in the presence of ADCA, UV light accelerated and visible light decelerated the reaction.