Coarse-grained model of conformation-dependent electrophoretic mobility and its influence on DNA dynamics.
Coarse-grained model of conformation-dependent electrophoretic mobility and its influence on DNA dynamics.
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构象依赖性电泳迁移率的粗粒度模型及其对 DNA 动力学的影响。
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Patrick T. Underhill
中科院分区：
文献类型：
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作者：
H. Pandey;Patrick T. Underhill
分子如λ-DNA的电泳迁移率取决于分子的构象。它已被证明，部分分子之间的电流体动力学相互作用导致的流动性，取决于构象，可以解释一些实验观察。我们已经开发了一个新的粗粒度模型，将这些变化的流动性到珠弹簧链模型。布朗动力学模拟已使用该模型进行。该模型再现的交叉流迁移时，发生在毛细管电泳的压力驱动的流动施加平行或反平行的电场。该模型还再现了当分子在拉伸场中被显著拉伸时迁移率的变化。我们发现，构象依赖的迁移率可以导致一种新的类型的解开的分子在强电场中。当分子的不同部分具有不同的迁移率并且电场很大时，就会发生这种情况。
The electrophoretic mobility of molecules such as λ-DNA depends on the conformation of the molecule. It has been shown that electrohydrodynamic interactions between parts of the molecule lead to a mobility that depends on conformation and can explain some experimental observations. We have developed a new coarse-grained model that incorporates these changes of mobility into a bead-spring chain model. Brownian dynamics simulations have been performed using this model. The model reproduces the cross-stream migration that occurs in capillary electrophoresis when pressure-driven flow is applied parallel or antiparallel to the electric field. The model also reproduces the change of mobility when the molecule is stretched significantly in an extensional field. We find that the conformation-dependent mobility can lead to a new type of unraveling of the molecule in strong fields. This occurs when different parts of the molecule have different mobilities and the electric field is large.