THE EFFECT OF GAMMA-IRRADIATION ON THE ENZYMATIC DEGRADATION OF POLYGLYCOLIC ACID ABSORBABLE SUTURES
THE EFFECT OF GAMMA-IRRADIATION ON THE ENZYMATIC DEGRADATION OF POLYGLYCOLIC ACID ABSORBABLE SUTURES
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DOI：
10.1002/jbm.820170612
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
WILLIAMS, DF
中科院分区：
文献类型：
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作者：
CHU, CC;WILLIAMS, DF
γ的影响对聚乙醇酸（PGA）缝线的酶降解和体内降解进行了检查。2-0号缝线在0- 2 - 0 mrad的剂量水平下接受辐照。本研究选择的3种酶是酯酶，α-胰凝乳蛋白酶和胰蛋白酶。将辐照缝线浸入酶溶液及其相应的缓冲液对照中，并植入近交系黑白白色hooded hister大鼠（利物浦品系）中。PGA缝线的降解通过机械方法确定。在研究的3种酶中，酯酶对未辐照和辐照PGA缝线的降解具有最高的酶促作用。直到20 mrad才观察到胰蛋白酶对PGA缝线的影响。胰蛋白酶的发现证实了这样一种假设，即最初对酶降解具有抗性的合成高分子量聚合物在改变其物理和化学结构后可能变得易于受到酶的攻击。与体外降解（pH = 7.5的0.1 M tris缓冲液）相比，植入的PGA缝线在体内降解中保持相似或略高的平均拉伸断裂强度;这些降解特征表明PGA在体内和体外降解中未表现出相似的行为。差异的程度取决于辐射剂量和降解持续时间，推测是由于酶对PGA缝线的构型和化学结构的特定作用。
The effect of .gamma. irradiation on the enzymatic as well as the in vivo degradation of polyglycolic acid (PGA) sutures was examined. The sutures of size 2-0 were irradiated at dosage levels of 0-20 mrad. The 3 enzymes chosen for this study were esterase, .alpha.-chymotrypsin and trypsin. The irradiated sutures were both immersed in the enzyme solutions, their corresponding buffer controls and implanted in inbred black-and-white hooded hister rats (Liverpool strain). The degradation of PGA sutures was determined mechanically. Among the 3 enzymes studied, esterase showed the highest enzymatic effect on the degradation of the unirradiated and irradiated PGA sutures. Trypsin''s effect on PGA sutures was not observed until 20 mrad. The findings of trypsin demonstrated the hypothesis that synthetic high molecular weight polymers, which are initially resistant to enzymatic degradation, could become prone to enzymatic attack after altering their physical and chemical structures. Implanted PGA sutures maintained a similar or slightly higher mean tensile breaking strength in in vivo degradation compared to in vitro degradation (0.1M tris buffer of pH = 7.5); these degradation profiles suggest that PGA does not display similar behavior in in vivo and in vitro degradations. The magnitude of dissimilarity depends on the radiation dosage and on the duration of degradation, and is speculated to be attributable to the specific action of enzymes with respect to the configuration and chemical structure of the PGA sutures.