S-allele identification by PCR analysis in sweet cherry cultivars
S-allele identification by PCR analysis in sweet cherry cultivars
复制标题
通过 PCR 分析鉴定甜樱桃品种中的 S 等位基因
DOI：
10.1111/j.1439-0523.2004.00984.x
复制
发表时间：
2004
期刊：
影响因子：
2
通讯作者：
J. Hormaza
中科院分区：
文献类型：
--
作者：
A. Wünsch;J. Hormaza
甜樱桃配子体自交不亲和性受S基因控制。因此，了解甜樱桃品种的S基因型对于通过确定亲和组合来建立高产果园是必不可少的。甜樱桃S-R酶的分离为利用不同的分子技术鉴定甜樱桃品种的S基因奠定了基础。以前，不亲和性群体的分配只能通过授粉试验在成熟的树木上进行。本工作根据甜樱桃S-R酶的保守序列设计引物，对71个甜樱桃品种进行了S等位基因分析，其中26个品种的S等位基因组成尚未见报道。该方法检测了未扩增的等位基因，鉴定了6个可能的S等位基因，定义了3个新的自交不亲和组，并建立了甜樱桃S等位基因分型的标准。
Gametophytic self-incompatibility, governed by the S-locus, operates in sweet cherry. The knowledge of the S-genotype of sweet cherry cultivars is therefore essential to establish productive orchards by defining compatible combinations. The isolation of sweet cherry S-R Nases has allowed the use of different molecular techniques to characterize the S-genotypes of sweet cherry cultivars. Previously, incompatibility group assignment could only be carried out on mature trees through pollination tests. In this work, PCR analysis with primers designed on the conserved sequences of sweet cherry S-R Nases has been used to characterize the S-genotype of 71 sweet cherry cultivars, including 26 cultivars whose S-allele constitution had not been previously described. This approach has allowed the detection of alleles that had not been amplified by PCR before, to identify six putative new S-alleles, to define three new self-incompatibility groups and to compile the standards for a PCR-based S-allele typing method in sweet cherry.