Association of Circulating Sclerostin With Bone Mineral Mass, Microstructure, and Turnover Biochemical Markers in Healthy Elderly Men and Women
Association of Circulating Sclerostin With Bone Mineral Mass, Microstructure, and Turnover Biochemical Markers in Healthy Elderly Men and Women
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DOI：
10.1210/jc.2013-2113
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发表时间：
2013-09-01
影响因子：
5.8
通讯作者：
Rizzoli, Rene
中科院分区：
文献类型：
--
作者：
Durosier, Claire;van Lierop, Antoon;Rizzoli, Rene
内容：Sclerostin抑制骨形成并参与骨对机械负荷的反应，但循环sclerostin的作用和意义知之甚少。目的：我们使用3种不同的免疫测定法评估血清sclerostin和促钙激素、骨转换标志物水平、骨矿物质含量/密度和微观结构之间的关系。设计、设置和参与者：在一项横断面研究中，对187名健康受试者的血清硬化素进行了测定。（98名女性; 89名男性）年龄65 +/- 1（+/- SD）岁。总体而言，试验I、II和III的平均硬化蛋白（95%置信区间）分别为37.3（18.0-69.2）ng/L、1165.8（464.0-2296.4）ng/L和513.5（250.7-950.9）ng/L。血清sclerostin是较高的男性与测定II和III。在所有3项测定中，硬化蛋白和PTH呈负相关，仅在调整全身骨矿物质含量（WB-BMC）后。调整性别和WB-BMC后，骨转换标志物I型胶原氨基末端前肽和I型胶原羧基末端交联端肽仅与测定II呈负相关。在所有3个测定，sclerostin呈正相关WB-BMC，远端桡骨和远端胫骨皮质面积，松质骨体积和小梁数量，腰椎和股骨近端面积骨密度调整后sexy.Conclusion：Sclerostin水平显着不同，根据免疫测定。与促钙激素或转换标志物的关联的检测依赖于免疫测定识别的表位和对骨量的调整。
Context: Sclerostin inhibits bone formation and is involved in the bone response to mechanical loading, but the role and significance of circulating sclerostin are poorly understood.Objective: We assessed the association between serum sclerostin and calcitropic hormones, bone turnover marker levels, bone mineral content/density, and microstructure using 3 different immunoassays.Design, Setting, and Participants: In a cross-sectional study, serum sclerostin was measured in a cohort of 187 healthy subjects (98 women; 89 men) aged 65 +/- 1 (+/- SD) years.Results: Overall, mean sclerostin (95% confidence interval) was 37.3 (18.0-69.2) ng/L, 1165.8 (464.0-2296.4) ng/L, and 513.5 (250.7-950.9) ng/L with assays I, II, and III, respectively. Serum sclerostin was higher in men with assays II and III. In all 3 assays, sclerostin and PTH were inversely correlated, only after adjustment for whole-body bone mineral content (WB-BMC). After adjustment for sex and WB-BMC, the bone turnover markers amino-terminal propeptide of type I rocollagen and carboxyterminal cross-linked telopeptide of type I collagen negatively correlated only with assay II. In all 3 assays, sclerostin positively correlated to WB-BMC, the distal radius and the distal tibia cortical area, cancellous bone volume and trabecular number, and lumbar spine and proximal femur areal bone mineral density after adjustment for sex.Conclusion: Sclerostin levels are markedly different according to the immunoassay used. Detection of an association with calcitropic hormones or turnover markers relies on the epitope recognized by the immunoassay and adjustment for bone mass.