Polymethylmethacrylate particles inhibit osteoblastic differentiation of bone marrow osteoprogenitor cells
Polymethylmethacrylate particles inhibit osteoblastic differentiation of bone marrow osteoprogenitor cells
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DOI：
10.1002/jbm.a.30697
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发表时间：
2006-06-15
影响因子：
4.9
通讯作者：
Goodman, Stuart B.
中科院分区：
文献类型：
--
作者：
Chiu, Richard;Ma, Ting;Goodman, Stuart B.
全关节置换术中无菌植入物松动通常是由颗粒介导的骨丢失引起的，这可能是骨溶解和骨形成受抑的共同作用。假体床中的骨再生取决于成骨细胞的活性及其在骨髓中从成骨细胞向成骨细胞的分化。本研究探讨了聚甲基丙烯酸甲酯(PMMA)颗粒对体外培养的骨髓成骨细胞分化能力的影响。小鼠骨髓细胞在成骨介质中分化的第一天，用PMMA颗粒攻击后，成骨细胞的增殖、碱性磷酸酶表达和矿化均呈剂量依赖性下降。未分化的骨髓细胞用PMMA颗粒在非成骨介质中处理5天，也显示出成骨能力的剂量依赖性丧失，并在随后的无颗粒成骨介质中持续生长。骨髓细胞在成骨介质中诱导分化后第5天加入PMMA颗粒后，细胞增殖明显下降，但碱性磷酸酶的表达和矿化作用不明显，表明骨髓细胞在分化的前5天对颗粒处理最为敏感。本研究表明，PMMA颗粒抑制了骨髓成骨祖细胞的成骨分化，这可能是假体周围骨丢失和种植失败的原因之一。(C)2006年威利期刊公司。
Aseptic implant loosening of total joint replacements often results from particle-mediated bone loss, which may be a combined effect of osteolysis and suppressed bone formation. Bone regeneration in the prosthetic bed depends on the activity of osteoblasts and their differentiation from osteoprogenitors in the bone marrow. This study investigated the effects of polymethylmethacrylate (PMMA) particles on the ability of bone marrow osteoprogenitors to differentiate into osteoblasts in vitro. Murine bone marrow cells challenged with PMMA particles on the first day of differentiation in osteogenic medium showed a dose-dependent decrease in osteoprogenitor proliferation, alkaline phosphatase expression, and mineralization. Undifferentiated bone marrow cells pretreated with PMMA particles in nonosteogenic medium for 5 days also showed a dose-dependent loss in osteogenic potential, which was sustained throughout subsequent growth in particle-free, osteogenic medium. Bone marrow cells challenged with PMMA particles after the fifth day of differentiation in osteogenic medium showed significant reductions in cellular proliferation, but not alkaline phosphatase expression and mineralization, indicating that bone marrow cells were most sensitive to particle treatment during the first 5 days of differentiation. This study demonstrated that PMMA particles inhibit osteoblastic differentiation of bone marrow osteoprogenitor cells, which may contribute to periprosthetic bone loss and implant failure. (c) 2006 Wiley Periodicals, Inc.