INCREASED NEUROPEPTIDE-Y (NPY)-LIKE IMMUNOREACTIVITY IN RAT SENSORY NEURONS FOLLOWING PERIPHERAL AXOTOMY
INCREASED NEUROPEPTIDE-Y (NPY)-LIKE IMMUNOREACTIVITY IN RAT SENSORY NEURONS FOLLOWING PERIPHERAL AXOTOMY
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DOI：
10.1016/0304-3940(91)90093-9
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发表时间：
1991-04-01
影响因子：
2.5
通讯作者：
BENNETT, GJ
中科院分区：
文献类型：
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作者：
WAKISAKA, S;KAJANDER, KC;BENNETT, GJ
使用免疫细胞化学检查外周轴突切开术（坐骨神经横断）对大鼠背根神经节（DRG）和脊髓灰质中神经肽 Y（NPY）的存在和分布的影响。 在正常大鼠和实验大鼠的假手术侧。 在腰脊髓的所有层中均观察到 NPY 样免疫反应性 (NPYir)，其中在背角 I-II 层中免疫染色轴突和轴突静脉曲张浓度特别高。 正常大鼠或实验大鼠假手术侧的 L4-L5 DRG 细胞中未检测到 NPYir。 轴索切断术后14天，神经损伤侧的同侧NPYir轴突密度大幅增加，腰脊髓出现静脉曲张；这在 III-V 层中尤其明显。 在同一只大鼠中，在切断神经一侧的 L4-L5 DRG 中的许多小、中和大神经元中观察到 NPYir。
The effects of peripheral axotomy (sciatic nerve transection) on the presence and distribution of neuropeptide Y (NPY) in rat dorsal root ganglion (DRG) and spinal grey matter were examined using immunocytochemistry. In normal rats and on the sham-operated side of experimental rats. NPY-like immunoreactivity (NPYir) was observed in all laminate of the lumbar spinal cord, with an especially dense concentration of immunostained axons and axonal varicosities in laminae I-II of the dorsal horn. There was no detectable NPYir in L4-L5 DRG cells from normal rats or from the sham-operated side of experimental rats. At 14 days after axotomy, there was a large ipsilateral increase in the density of NPYir axons and varicosities in the lumbar spinal cord on the side of the nerve injury; this was especially apparent in laminae III-V. In the same rats, NPYir was observed in many small, medium, and large neurons in the L4-L5 DRGs on the side of the severed nerve.