Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein
Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein
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DOI：
10.1007/978-1-0716-0954-5_16
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发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Zhang, Chunhua
中科院分区：
文献类型：
--
作者：
Li, Xiaohui;Zhang, Chunhua
Differential scanning fluorimetry (DSF) can be used to detect the binding of a small molecule ligand to a purified target protein. Upon binding with certain ligands, the protein can be stabilized from thermal denaturation. DSF uses a fluorescent dye and Real-Time PCR instrument to detect the unfolding process of proteins during thermal denaturation. The experiment can be set up and finished in 1 day once the purified protein is available.
