Expression of nitrogen transporter genes in roots of winter wheat (Triticum aestivum L.) in response to soil drought with contrasting nitrogen supplies
Expression of nitrogen transporter genes in roots of winter wheat (Triticum aestivum L.) in response to soil drought with contrasting nitrogen supplies
复制标题
DOI：
10.1071/cp15152
复制
发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Gao, Yajun
中科院分区：
文献类型：
--
作者：
Duan, Jianfeng;Tian, Hui;Gao, Yajun
干旱胁迫下冬小麦根中硝酸盐和铵转运蛋白基因的表达在很大程度上未知。利用温室试验研究了5个硝酸盐转运蛋白（NR 7）基因（TaNRT 2. 1、TaNRT 2. 2、TaNRT 2. 3、TaNRT 1. 1、TaNRT 1. 2）和3个铵离子转运蛋白（AMY）基因（TaAAIT 1. 2、TaNRT 2. 3）在水稻中的表达。1、TaAMT1.2、TaAA1T2。1)在有限氮（N）（不加N）和充足氮（加0.3g N kg/土）条件下，冬小麦根系对土壤干旱的响应。采用氮吸收效率低和高的两种小麦基因型，在小麦营养生长期和生殖生长期进行水分胁迫处理。使用实时逆转录PCR定量所有基因的表达。结果表明，生长在氮素充足土壤上的小麦植株比生长在氮素有限土壤上的小麦植株对干旱胁迫更敏感。ART和AMT基因表达对土壤干旱的响应主要取决于施氮量、小麦基因型和生育期。干旱胁迫主要诱导了氮低效基因型XY 6中两个低亲和力NR T基因（TaNRT 1.1和TaNRT 1.2）的表达，而干旱胁迫则抑制了氮高效基因型XY 107中这两个基因的表达。高亲和NRT基因TaNRT2.1的表达受到干旱胁迫的抑制，但另外两个高亲和NRT基因TaNRT2.2和TaNRT2.3的表达受到土壤干旱的诱导或抑制，这取决于施氮量和生育期。TaAMT 1. 2基因的表达受干旱胁迫的诱导，而TaAMT 1. 1和TaAVIT 2. 1基因的表达受干旱胁迫的抑制。TaNRT 2.1在XY 107中的表达显著高于XY 6。
The expression of nitrate and ammonium transporter genes in the roots of winter wheat in response to drought stress is largely unknown. A greenhouse experiment was established to study the expression of five putative nitrate transporter (NR7) genes (TaNRT2.1, TaNRT2.2,TaNRT2.3,TaNRT1.1,TaNRT1.2) and three ammonium transporter (AMY) genes (TaAAIT1. 1, TaAMT1.2, TaAA1T2. 1) in the roots of winter wheat in response to soil drought under conditions of limited nitrogen (N) (no N added) and adequate N (addition of 0.3 g N kg I soil). Two wheat genotypes with low and high N-uptake efficiencies were used, and water-stress treatments were applied at the vegetative and reproductive growth stages of wheat. Expression of all of the genes was quantified using real-time reverse transcription PCR. The results indicated that wheat plants growing in the N-adequate soil were more sensitive to drought stress than those growing in the N-limited soil. The response of the expression of the ART and AMT genes to soil drought largely depends on N application, wheat genotype and growth stage. The expression of the two low-affinity NR T genes (i.e. TaNRT1.1 and TaNRT1.2) in the N-inefficient genotype XY6 was mainly induced by drought stress, but the expression of the two genes in the N-efficient genotype XY107 was repressed by drought stress. The expression of the high-affinity NRTgene TaNRT2.1 was repressed by drought stress, but the expression of the other two high-affinity NRT genes, TaNRT2.2 and TaNRT2.3, was induced or repressed by soil drought depending on N application and growth stage. The expression of the genes TaA,VITI.1 and TaA,VIT2.1 was mainly repressed by drought stress, whereas the expression of the gene TaAMT1.2 was induced by drought stress. The expression of TaNRT2.1 in XY 107 was significantly higher than in XY6.