Trichosanthin down-regulates Notch signaling and inhibits proliferation of the nasopharyngeal carcinoma cell line CNE2 in vitro
Trichosanthin down-regulates Notch signaling and inhibits proliferation of the nasopharyngeal carcinoma cell line CNE2 in vitro
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DOI：
10.1016/j.fitote.2012.02.011
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发表时间：
2012-07-01
期刊：
影响因子：
3.4
通讯作者：
Yu, Shudong
中科院分区：
文献类型：
--
作者：
Liu, Fenye;Wang, Bing;Yu, Shudong
鼻咽癌（nasopharyngeal carcinoma，NPC）的预后一直较差。天花粉蛋白（Trichosanthin，TCS）具有流产、抗病毒、免疫调节及多种抗肿瘤药理活性，但其对鼻咽癌的作用尚未见报道，其确切的抑瘤机制也不清楚。本研究通过体外实验观察TCS对CNE 2细胞增殖、凋亡及软琼脂集落形成能力的影响。此外，还检测了TCS和对照细胞中Notch信号通路的激活状态。结果表明，天花粉蛋白对鼻咽癌细胞株CNE 2有明显的抑制作用，能降低CNE 2细胞的克隆形成能力，并诱导CNE 2细胞凋亡。下调Notch信号可能是TCS抑制NPC的机制之一。(C)2012爱思唯尔有限公司版权所有。
The prognosis of nasopharyngeal carcinoma (NPC) is still poor. Trichosanthin (TCS) has abortifacient, anti-virus, immunoregulation and various anti-tumor pharmacological activities, but there are no reports about its effect on NPC and the exact mechanisms that TCS inhibits tumor are not well known. In this study, the proliferation, apoptosis and soft agar colony formation abilities of CNE2 cells were examined with various assays in vitro followed by treatment with TCS. Furthermore, the activation status of Notch signaling pathway in TCS and control cells also was examined. The results revealed that TCS could inhibit NPC cell line CNE2 in vitro, reduce clone formation ability and induce apoptosis of CNE2 cells. Down-regulation of Notch signaling may be one of the mechanisms that TCS inhibits NPC. (C) 2012 Elsevier B.V. All rights reserved.