Microbial Eukaryote Diversity and Activity in the Water Column of the South China Sea Based on DNA and RNA High Throughput Sequencing.
Microbial Eukaryote Diversity and Activity in the Water Column of the South China Sea Based on DNA and RNA High Throughput Sequencing.
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基于DNA和RNA高通量测序的南海水体微生物真核生物多样性和活性
DOI：
10.3389/fmicb.2017.01121
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发表时间：
2017
影响因子：
5.2
通讯作者：
Warren A
中科院分区：
文献类型：
--
作者：
Xu D;Li R;Hu C;Sun P;Jiao N;Warren A
为研究南海水体中微生物真核生物的多样性和代谢活性，从两个站位的深海样品中共提取基因组DNA和RNA。扩增SSU rRNA基因及其转录本（cDNA）的V9区，并使用高通量测序进行测序。我们的研究显示：（1）DNA和RNA数据集在微生物真核生物群落组成上表现出显著差异，同一样品的两个数据集之间的变异性超过了每个数据集内样品之间的变异性，表明核酸来源在决定微真核生物群落组成上压倒了环境因素;（2）尽管两个数据集之间的群落组成存在差异，但DNA和RNA都揭示了微生物真核生物相似的深度相关分布模式;（3）使用RNA：以DNA作为相对代谢活性的代用指标，发现部分而非全部微生物真核生物类群的相对代谢活性存在深度相关模式，具有深度相关模式的类群通常在中层水体中代谢活性最高;和（4）证实了深海中存在活的和活跃的光合自养微生物真核生物，表明它们在控制深海有机碳库方面起着重要作用。总的来说，我们的研究揭示了热带贫营养海洋水体中微生物真核生物的多样性和活性，以及它们通过生物泵将有机物质从表层海洋向下运输到深层的潜在贡献。
To study the diversity and metabolic activity of microbial eukaryotes in the water column of the South China Sea, genomic DNA and RNA were co-extracted from samples collected down to bathyal depth at two sites. V9 regions of both SSU rRNA gene and its transcript (cDNA) were amplified and sequenced using high throughput sequencing. Our study revealed: (1) DNA and RNA datasets showed significant differences in microbial eukaryote community composition, with the variability between the two datasets for the same sample exceeding that between samples within each dataset, indicating that nucleic acid source overrode environmental factors in determining the composition of microeukaryotes; (2) despite the differences in community composition between the two datasets, both DNA and RNA revealed similar depth-related distribution patterns of microbial eukaryotes; (3) using the ratio of RNA: DNA as a proxy of relative metabolic activity, a depth-related pattern was found for the relative metabolic activity of some but not all groups of microbial eukaryotes, with the highest activity for the groups with depth-related pattern usually found in the middle water layers; and (4) the presence of live and active photoautotrophic microbial eukaryotes in the deep ocean was confirmed, indicating that they play an important role in controlling the deep-sea organic carbon pool. Overall, our study sheds light on the diversity and activity of microbial eukaryotes in the water column of a tropical oligotrophic ocean and their potential contributions in the downward transportation of organic material from the surface ocean to the deep via the biological pump.