Dorsal Root Ganglionic Field Stimulation Relieves Spontaneous and Induced Neuropathic Pain in Rats
Dorsal Root Ganglionic Field Stimulation Relieves Spontaneous and Induced Neuropathic Pain in Rats
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DOI：
10.1016/j.jpain.2016.09.004
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发表时间：
2016-12-01
期刊：
影响因子：
4
通讯作者：
Hogan, Quinn H.
中科院分区：
文献类型：
--
作者：
Pan, Bin;Yu, Hongwei;Hogan, Quinn H.
背根神经节（DRG）电刺激（神经节场刺激[GFS]）可有效缓解临床疼痛，但其机制尚不清楚。因此，我们开发了一种大鼠模型的GFS测试镇痛作用的背景下，神经病理性疼痛。在L4用双极电极施加GFS，使用复制临床使用的参数（20 Hz，150 μ s脉冲宽度，电流为运动阈值的80%）。神经病理性疼痛由胫神经损伤（TNI）产生。通过确定从点状机械刺激中退缩的阈值，通过识别对有害机械刺激的痛觉过敏反应和对冷的超敏反应来监测疼痛行为。疼痛的情感维度采用条件性位置偏爱测量。我们发现电极插入没有引起疼痛的行为证据，也没有产生DRG损伤的组织学证据。GFS逆转TNI诱导的对冷和机械痛觉过敏和异常性疼痛的超敏反应。在GFS后15分钟，异常性疼痛仍然减少。条件性位置偏爱实验表明，GFS在未受伤的对照组动物中没有奖励作用，但在TNI组动物中有奖励作用，这揭示了GFS对自发性疼痛的镇痛作用。我们的结论是，GFS减轻大鼠神经病理性疼痛。这个模型可以提供一个平台，用于识别机制和新的应用GFS.Perspective：我们表明，电刺激大鼠背根神经节逆转神经性疼痛的行为，并提供了一个奖励的效果，自发性神经性疼痛的动物。这证实了DRG刺激在动物模型中的镇痛功效，并为临床前探索提供了平台。(C)2016年美国疼痛协会American Pain Society
Dorsal root ganglion (DRG) electrical stimulation (ganglionic field stimulation [GFS]) is effective in relieving clinical pain, but its mechanism is unknown. We therefore developed a rat model for GFS to test analgesic effects in the context of neuropathic pain. GFS was applied with a bipolar electrode at L4, using parameters replicating clinical use (20 Hz, 150-mu s pulse width, current at 80% of motor threshold). Neuropathic pain was generated by tibial nerve injury (TNI). Pain behavior was monitored by determining the threshold for withdrawal from punctate mechanical stimuli, by identifying hyperalgesic responses to noxious mechanical stimuli, and by hypersensitivity to cold. The affective dimension of pain was measured using conditioned place preference. We found that electrode insertion caused no behavioral evidence of pain and produced no histological evidence of DRG damage. GFS reversed TNI-induced hypersensitivity to cold and mechanical hyperalgesia and allodynia. Allodynia remained diminished 15 minutes after GFS. Conditioned place preference showed that GFS was not rewarding in uninjured control animals but was rewarding in animals subjected to TNI, which reveals analgesic efficacy of GFS for spontaneous pain. We conclude that GFS relieves neuropathic pain in rats. This model may provide a platform for identifying mechanisms and novel applications of GFS.Perspective: We show that electrical stimulation of the DRG in rats reverses neuropathic pain behavior and provides a rewarding effect to animals with spontaneous neuropathic pain. This confirms analgesic efficacy of DRG stimulation in an animal model, and provides a platform for preclinical exploration. (C) 2016 by the American Pain Society