A Novel Human Gal-3-O-Sulfotransferase
A Novel Human Gal-3-O-Sulfotransferase
复制标题
一种新型人类 Gal-3-O-磺基转移酶
DOI：
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发表时间：
2001
影响因子：
4.8
通讯作者：
T. Muramatsu
中科院分区：
文献类型：
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作者：
F. El;K. Uchimura;R. Kannagi;T. Muramatsu
基于与已克隆的人谷胱甘肽磺基转移酶（CST）cDNA序列的同源性，从人胎脑cDNA文库中筛选出一个新的CST。新的磺基转移酶基因存在于人染色体11 q13上，其位置不同于人CST，也不同于最近克隆的人β-Gal 3′-磺基转移酶（GP 3ST）。分离的cDNA包含一个开放阅读框架，编码一个预测的蛋白质的431个氨基酸残基与II型跨膜拓扑结构。其氨基酸序列与人CST的同源性为33%，与人GP 3ST的同源性为38%。在中国仓鼠卵巢细胞中表达的重组酶催化Galβ1-4GlcNAc中非还原性β-半乳糖残基3位的硫酸酯转移。2型链作为良好的受体，而1型链作为不良受体，并且发现对Galβ1-3GalNAc的中间活性。因此，底物特异性不同于GP 3ST。在新克隆的酶中未检测到CST活性。北方印迹分析表明，磺基转移酶mRNA在甲状腺中强表达，在脑、心脏、肾脏和脊髓中中度表达。将该酶cDNA和岩藻糖基转移酶III共转染COS-7细胞，导致（SO 4 -3）Galβ1-4（Fucα1-3）GlcNAc和少量（SO 4 -3）Galβ1-3（Fucα1-4）GlcNAc的表达。这些结果表明，新克隆的酶是一种新的Gal-3-O-磺基转移酶，参与（SO 4 -3）Galβ1-4（Fucα1-3）GlcNAc结构的生物合成。
Based on sequence homology with the previously cloned human cerebroside sulfotransferase (CST) cDNA, a novel sulfotransferase was cloned by screening a human fetal brain cDNA library. The novel sulfotransferase gene was present on human chromosome 11q13; the location was different from human CST and from that of the recently cloned human β-Gal 3′-sulfotransferase (GP3ST). The isolated cDNA contained an open reading frame that encoded a predicted protein of 431 amino acid residues with type II transmembrane topology. The amino acid sequence showed 33% identity with that of human CST and 38% with that of human GP3ST. The recombinant enzyme expressed in Chinese hamster ovary cells catalyzed transfer of sulfate to position 3 of non-reducing β-galactosyl residues in Galβ1–4GlcNAc. Type 2 chains served as good acceptors, whereas type 1 chains served as poor acceptors, and intermediate activity was found toward Galβ1–3GalNAc. Therefore, the substrate specificity was different from that of GP3ST. CST activity was not detected in the newly cloned enzyme. Northern blotting analysis showed that the sulfotransferase mRNA was strongly expressed in the thyroid and moderately expressed in the brain, heart, kidney, and spinal cord. Co-transfection of the enzyme cDNA and fucosyltransferase III into COS-7 cells resulted in expression of (SO4-3)Galβ1–4(Fucα1–3)GlcNAc and a small amount of (SO4-3)Galβ1–3(Fucα1–4)GlcNAc. These results indicated that the newly cloned enzyme is a novel Gal-3-O-sulfotransferase and is involved in biosynthesis of the (SO4-3)Galβ1–4(Fucα1–3)GlcNAc structure.
甲状腺球蛋白的天冬酰胺连接的复合碳水化合物单元中存在硫酸盐。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Spiro,RG;Bhoyroo,VD
通讯作者：
Bhoyroo,VD
负责 N 连接碳水化合物单元中末端 β-D-半乳糖基残基 3-O-硫酸化的甲状腺磺基转移酶的表征。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kato,Y;Spiro,RG
通讯作者：
Spiro,RG