Kinetoplast DNA maxicircles: networks within networks.
Kinetoplast DNA maxicircles: networks within networks.
复制标题
动质体 DNA 大环：网络中的网络。
DOI：
10.1073/pnas.90.16.7809
复制
发表时间：
1993
影响因子：
11.1
通讯作者：
Shapiro,TA
中科院分区：
文献类型：
--
作者：
Shapiro,TA
动基体DNA（kDNA）是锥虫的线粒体DNA，是一个巨大的互锁小环和大环网络。我们选择性地删除小环从马锥虫kDNA网络的限制性内切酶切割。大环保持在聚合物中，对蛋白酶或RNA酶具有抗性，并且不含残留的微环，但通过拓扑异构酶II分解成环状单体。因此，大环在kDNA网络中形成独立的索链。大环索烃的大小、组成和组织的异质性反映了kDNA复制过程中发生的变化。大环索烃的玫瑰花状排列明显不同于小环索烃。锥虫kDNA网络揭示了独特的拓扑结构复杂性：它们由完全不同的链烷组成，这些链烷又彼此广泛互锁。
Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomes, is an enormous network of interlocked minicircles and maxicircles. We selectively removed minicircles from Trypanosoma equiperdum kDNA networks by restriction enzyme cleavage. Maxicircles remained in aggregates that were resistant to protease or RNase and contained no residual minicircles, but were resolved into circular monomers by topoisomerase II. Maxicircles thus form independent catenanes within kDNA networks. Heterogeneity in the size, composition, and organization of maxicircle catenanes reflects changes that occur during kDNA replication. The rosette-like arrangement of maxicircle catenanes is distinctly different from that of minicircle catenanes. Trypanosome kDNA networks reveal unique topological complexity: they are composed of entirely dissimilar catenanes that are in turn extensively interlocked with one another.